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Anti-NODAL Antibody (C-Terminus) LS-C116663

Catalog Size Price
LS-C116663-50 50 µg Unavailable

Related Products

14 NODAL Antibodies

Most Popular NODAL Antibodies

Anti-NODAL Antibody (aa279-289) LS-C55181
Goat Polyclonal to Human NODAL
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig
Peptide Enzyme-Linked Immunosorbent Assay
Unconjugated
Anti-NODAL Antibody (aa242-253) IHC-plus™ LS-B3955
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Human, Monkey, Bat, Bovine, Dog, Horse, Pig
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Anti-NODAL antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3955 concentration 3.75 ug/ml.
Anti-NODAL Antibody (clone 5C3) LS-C133350
Mouse Monoclonal [clone 5C3] (IgG1,k) to Human NODAL
Human, Mouse, Rat
IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
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Immunoperoxidase of monoclonal antibody to NODAL on formalin-fixed paraffin-embedded human endometrium cancer. [antibody concentration 3 ug/ml]
Anti-NODAL Antibody LS-C356605
Rabbit Polyclonal to Human NODAL
Human
Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Unconjugated
Anti-NODAL Antibody at 1:1000 dilution + HepG2 whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 40 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-NODAL Antibody (C-Terminus) LS-C413386
Rabbit Polyclonal to Human NODAL
Human
IHC, IHC - Paraffin, Western blot
Unconjugated
Immunohistochemical analysis of NODAL staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

100% Guaranteed
Rabbit Polyclonal to Human NODAL
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse
Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human NODAL
Rabbit
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse (tested or 100% immunogen sequence identity)
Rabbit (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

NODAL antibody was raised against synthetic peptide from C-Terminus of human NODAL (Q96S42, NP_060525). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Bovine, Bat, Horse, Guinea pig (100%); Galago, Elephant, Rabbit (92%).
LS-E20822 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C116663. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
C-Terminus
Human NODAL
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Packaging

Lyophilized from PBS with 2% sucrose
Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
For research use only.

About NODAL

Q96S42 NM_018055 NP_060525.3

NODAL Antibody, HTX5 Antibody, Nodal homolog Antibody, Nodal, mouse, homolog Antibody, Nodal homolog (mouse) Antibody

Essential for mesoderm formation and axial patterning during embryonic development.

Western blot

NODAL antibody LS-C116663 Western blot of THP-1 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
NODAL antibody LS-C116663 Western blot of THP-1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 5/27/2018

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