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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NOD2 / CARD15 Antibody (aa28‑301, clone 2D9, Biotin) LS‑C141210
CARD15 antibody LS-C141210 is a biotin-conjugated mouse monoclonal antibody to CARD15 (NOD2) from human and mouse. Validated for IHC, IP and WB.
Catalog
Size
Price
LS-C141210-100
100 µl (0.58 mg/ml)
Unavailable

Popular NOD2 / CARD15 Products

Detection of endogenous NOD2 in 50 ug of HT29 cell lysate. ECL detection in 15 minutes.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse
Applications: IHC
Antibody (0.1 ug/ml) staining of Human Peripheral Blood Mononucleocyte lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Species: Human, Hamster
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Anti-NOD2 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2720 concentration 3 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Western blot, ELISA
Anti-NOD2 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2721 concentration 3 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Western blot, ELISA
Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation

Product Description

CARD15 antibody LS-C141210 is a biotin-conjugated mouse monoclonal antibody to CARD15 (NOD2) from human and mouse. Validated for IHC, IP and WB.
About NOD2 / CARD15
Recognizes muramyl dipeptide (MDP) constituents of bacterial peptidoglycans and plays a key role in gastrointestinal immunity: upon stimulation, binds the proximal adapter receptor-interacting RIPK2, which recruits ubiquitin ligases as XIAP, BIRC2, BIRC3 and the LUBAC complex, triggering activation of MAP kinases and activation of NF-kappa-B signaling, leading to activate the transcription of hundreds of genes involved in immune response. Q9HC29 NM_022162 NP_071445.1

NOD2 Antibody, BLAU Antibody, CARD15 Antibody, ACUG Antibody, NOD-like receptor C2 Antibody, NOD2B Antibody, NLRC2 Antibody, IBD1 Antibody, CLR16.3 Antibody, PSORAS1 Antibody


Specifications

Target
Human NOD2 / CARD15
Host
Mouse
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [2D9]
Conjugations
Biotin. Also available Unconjugated or conjugated with DY550, DY650, DY488.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
  • Immunoprecipitation
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
NOD2 / CARD15 antibody was raised against recombinant human NOD2 protein corresponding to residues 28-301
Epitope
aa28-301
Specificity
NOD2 protein.
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, 0.05% sodium azide
Storage
Store at 4°C. Do not freeze.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Detection of endogenous NOD2 in 50 ug of HT29 cell lysate. ECL detection in 15 minutes.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of endogenous NOD2 in 50 ug of HT29 cell lysate. ECL detection in 15 minutes. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of NOD2 in 20 ug of NOD2 transfected 293T cell lysate. ECL detection in 10 seconds.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of NOD2 in 20 ug of NOD2 transfected 293T cell lysate. ECL detection in 10 seconds. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of endogenous NOD2 in 50 ug of HT29 cell lysate. ECL detection in 15 minutes.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of endogenous NOD2 in 50 ug of HT29 cell lysate. ECL detection in 15 minutes. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of NOD2 in 20 ug of NOD2 transfected 293T cell lysate. ECL detection in 10 seconds.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of NOD2 in 20 ug of NOD2 transfected 293T cell lysate. ECL detection in 10 seconds. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of endogenous NOD2 in 50 ug of HT29 cell lysate. ECL detection in 15 minutes.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of endogenous NOD2 in 50 ug of HT29 cell lysate. ECL detection in 15 minutes. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of NOD2 in 20 ug of NOD2 transfected 293T cell lysate. ECL detection in 10 seconds.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of NOD2 in 20 ug of NOD2 transfected 293T cell lysate. ECL detection in 10 seconds. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of endogenous NOD2 in 50 ug of HT29 cell lysate. ECL detection in 15 minutes.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of endogenous NOD2 in 50 ug of HT29 cell lysate. ECL detection in 15 minutes. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of NOD2 in 20 ug of NOD2 transfected 293T cell lysate. ECL detection in 10 seconds.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of NOD2 in 20 ug of NOD2 transfected 293T cell lysate. ECL detection in 10 seconds. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/20/2018

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