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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NLRP2 / NALP2 Antibody (aa1037‑1050) LS‑C112984
NALP2 antibody LS-C112984 is an unconjugated goat polyclonal antibody to human NALP2 (NLRP2). Validated for Peptide-ELISA and WB.
Catalog
Size
Price
LS-C112984-100
100 µg
Unavailable

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Product Description

NALP2 antibody LS-C112984 is an unconjugated goat polyclonal antibody to human NALP2 (NLRP2). Validated for Peptide-ELISA and WB.
About NLRP2 / NALP2
Suppresses TNF- and CD40-induced NFKB1 activity at the level of the IKK complex, by inhibiting NFKBIA degradation induced by TNF. When associated with PYCARD, activates CASP1, leading to the secretion of mature proinflammatory cytokine IL1B. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases. Q9NX02 NM_017852 NP_060322.1

NLRP2 Antibody, CLR19.9 Antibody, PAN1 Antibody, NALP2 Antibody, NBS1 Antibody, PYPAF2 Antibody


Specifications

Target
Human NLRP2 / NALP2
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.3 - 1 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
Immunogen
NLRP2 / NALP2 antibody was raised against synthetic peptide C-KNPQLIIDTEKHHP from the C-terminus (near) of human NLRP2 / NALP2 (NP_060322.1). Percent identity by BLAST analysis: Human, Gorilla (100%); Gibbon, Monkey (86%).
Blocking Peptide
LS-E27304 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C112984. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa1037-1050
Specificity
Human NLRP2 / NALP2.
Usage
Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 140kDa band observed in lysates of cell line K562 (calculated MW of 121kDa according to NP_060322.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1 ug/ml.
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human NLRP2 with DYKDDDDK tag probed with (1ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/5000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Primary
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human NLRP2 with DYKDDDDK tag probed with (1ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/5000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Primary

Western blot

NLRP2 antibody (0.5 ug/ml) staining of K562 lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
NLRP2 antibody (0.5 ug/ml) staining of K562 lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human NLRP2 with DYKDDDDK tag probed with (1ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/5000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Primary
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human NLRP2 with DYKDDDDK tag probed with (1ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/5000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Primary

Western blot

NLRP2 antibody (0.5 ug/ml) staining of K562 lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
NLRP2 antibody (0.5 ug/ml) staining of K562 lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human NLRP2 with DYKDDDDK tag probed with (1ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/5000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Primary
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human NLRP2 with DYKDDDDK tag probed with (1ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/5000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Primary

Western blot

NLRP2 antibody (0.5 ug/ml) staining of K562 lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
NLRP2 antibody (0.5 ug/ml) staining of K562 lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human NLRP2 with DYKDDDDK tag probed with (1ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/5000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Primary
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human NLRP2 with DYKDDDDK tag probed with (1ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/5000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Primary

Western blot

NLRP2 antibody (0.5 ug/ml) staining of K562 lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
NLRP2 antibody (0.5 ug/ml) staining of K562 lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.


Requested From: 
Date Requested: 6/23/2018

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