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Anti-NKX3-1 Antibody (N-Terminus) IHC-plus™ LS-B3435

Note: This antibody replaces LS-C25772


Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular NKX3-1 Antibodies

Anti-NKX3-1 Antibody (N-Terminus) IHC-plus™ LS-B3435
Mouse Monoclonal (IgG1) to Human NKX3-1
IHC - Paraffin, IHC - Frozen, Western blot, ELISA
Immunohistochemistry Image
Anti-NKX3-1 Antibody (aa118-145) IHC-plus™ LS-B11115
Rabbit Polyclonal to Human NKX3-1
IHC - Paraffin, Western blot, Flow Cytometry
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed Publications Publications (1)
Mouse Monoclonal (IgG1) to Human NKX3-1
IHC - Paraffin, IHC - Frozen, Western blot, ELISA


Human NKX3-1
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal
Protein A purified


  • IHC - Paraffin (10 µg/ml)
  • IHC - Frozen
  • Western blot (1 - 3 µg/ml)
  • ELISA (0.1 - 1 µg/ml)

Specificity and Use

NKX3-1 antibody was raised against fusion protein containing the N-terminal domain of the human Nkx3.1 protein.
Reacts with human Nkx3.1 protein, and does not cross-react with mouse. On Western blots, this antibody detects a band at ~34kD. Reactivity has been confirmed with human LN Cap-FGC prostate carcinoma cell lysates.
Immunohistochemistry: LS-B3435 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3435 was determined to be 10 ug/ml.


PBS, pH 7.4, 0.1% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

Publications (1)

NKX3.1 haploinsufficiency is prognostic for prostate cancer relapse following surgery or image-guided radiotherapy. Locke JA, Zafarana G, Ishkanian AS, Milosevic M, Thoms J, Have CL, Malloff CA, Lam WL, Squire JA, Pintilie M, Sykes J, Ramnarine VR, Meng A, Ahmed O, Jurisica I, van der Kwast T, Bristow RG. Clinical cancer research : an official journal of the American Association for Cancer Research. 2014 Jan;18:308-16. [Full Text Article] [PubMed:22048240]

About NKX3-1

Q99801 NM_006167 NP_006158.2

NKX3-1 Antibody, BAPX2 Antibody, NK homeobox, family 3, A Antibody, NK3 homeobox 1 Antibody, NKX3 Antibody, NKX3.1 Antibody, Homeobox protein Nkx-3.1 Antibody, NKX3A Antibody

Transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. Plays an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. Acts as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to inhibit proliferation and invasion activities of PC-3 prostate cancer cells.


Anti-NKX3-1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3435 concentration 10 ug/ml.


Anti-NKX3-1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3435 concentration 10 ug/ml.

Requested From: United States
Date Requested: 10/25/2016

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