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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NKX2-1 / Thyroid-Specific TF Antibody (aa1-30) IHC-plus™ LS-B10666

Note: This antibody replaces LS-C164895
Thyroid-Specific TF antibody LS-B10666 is an unconjugated rabbit polyclonal antibody to human Thyroid-Specific TF (NKX2-1). Validated for Flow, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B10666-200
200 µl
$425
Description
Thyroid-Specific TF antibody LS-B10666 is an unconjugated rabbit polyclonal antibody to human Thyroid-Specific TF (NKX2-1). Validated for Flow, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human NKX2-1 / Thyroid-Specific TF
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E450 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B10666. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa1-30
Specificity
This NKX2-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human NKX2-1.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
About NKX2-1 / Thyroid-Specific TF
P43699 NM_003317 NP_003308.1

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Images

Immunohistochemistry

Anti-TTF1 / TTF-1 / NKX2-1 antibody IHC staining of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-TTF1 / TTF-1 / NKX2-1 antibody IHC staining of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

Western blot

NKX2-1 Antibody western blot of HeLa cell line lysates (35 ug/lane). The NKX2-1 antibody detected the NKX2-1 protein (arrow).
NKX2-1 Antibody western blot of HeLa cell line lysates (35 ug/lane). The NKX2-1 antibody detected the NKX2-1 protein (arrow).

Flow Cytometry

NKX2-1 Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
NKX2-1 Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

Anti-TTF1 / TTF-1 / NKX2-1 antibody IHC staining of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-TTF1 / TTF-1 / NKX2-1 antibody IHC staining of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

Western blot

NKX2-1 Antibody western blot of HeLa cell line lysates (35 ug/lane). The NKX2-1 antibody detected the NKX2-1 protein (arrow).
NKX2-1 Antibody western blot of HeLa cell line lysates (35 ug/lane). The NKX2-1 antibody detected the NKX2-1 protein (arrow).

Flow Cytometry

NKX2-1 Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
NKX2-1 Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

Anti-TTF1 / TTF-1 / NKX2-1 antibody IHC staining of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-TTF1 / TTF-1 / NKX2-1 antibody IHC staining of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

Western blot

NKX2-1 Antibody western blot of HeLa cell line lysates (35 ug/lane). The NKX2-1 antibody detected the NKX2-1 protein (arrow).
NKX2-1 Antibody western blot of HeLa cell line lysates (35 ug/lane). The NKX2-1 antibody detected the NKX2-1 protein (arrow).

Flow Cytometry

NKX2-1 Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
NKX2-1 Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

Anti-TTF1 / TTF-1 / NKX2-1 antibody IHC staining of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-TTF1 / TTF-1 / NKX2-1 antibody IHC staining of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

Western blot

NKX2-1 Antibody western blot of HeLa cell line lysates (35 ug/lane). The NKX2-1 antibody detected the NKX2-1 protein (arrow).
NKX2-1 Antibody western blot of HeLa cell line lysates (35 ug/lane). The NKX2-1 antibody detected the NKX2-1 protein (arrow).

Flow Cytometry

NKX2-1 Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
NKX2-1 Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Requested From: United States
Date Requested: 4/23/2019
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