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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NKX1‑1 Antibody (aa203‑252) LS‑C31278
NKX1-1 antibody LS-C31278 is an unconjugated rabbit polyclonal antibody to NKX1-1 from human, mouse and guinea pig. Validated for WB.
Catalog
Size
Price
LS-C31278-100
100 µg
$325

Popular NKX1-1 Products

Fluorescent confocal image of HeLa cell stained with NKX1-1 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NKX1-1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NKX1-1 immunoreactivity is localized to Nucleus significantly.
Species: Human
Applications: Immunofluorescence, Western blot
Antibody
Species: Human
Applications: Western blot, ELISA
Antibody
Species: Human
Applications: Western blot, ELISA
Antibody
Species: Human
Applications: Western blot, ELISA
Antibody
Species: Human
Applications: Western blot, ELISA

Product Description

NKX1-1 antibody LS-C31278 is an unconjugated rabbit polyclonal antibody to NKX1-1 from human, mouse and guinea pig. Validated for WB.
XM_926341

NKX1-1 Antibody, Homeobox protein 153 Antibody, HPX153 Antibody, Homeobox protein SAX-2 Antibody, NK1 homeobox 1 Antibody, HPX-153 Antibody, HPX-153 homeobox Antibody, NKX-1.1 Antibody, HSPX153 Antibody, SAX2 Antibody


Specifications

Target
Mouse NKX1-1
Host
Rabbit
Reactivity
Mouse, Human, Guinea pig (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot (1.25 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
NKX1-1 antibody was raised against synthetic peptide located between aa203-252 of mouse NKX1-1. Percent identity by BLAST analysis: Human, Mouse (100%).
Blocking Peptide
LS-E13500 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C31278. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa203-252
Specificity
Mouse NKX1-1
Presentation
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Reconstitution
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.

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Images

Western blot

SP2/0 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SP2/0 Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

SP2/0 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SP2/0 Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

SP2/0 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SP2/0 Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

SP2/0 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SP2/0 Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 9/19/2018

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