Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone Y4E6] (IgG1) to Human NKP30
Western blot, ELISA
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [Y4E6]
Protein G purified
Western blot (1:500 - 1:2000)
IHC - Paraffin
Specificity and Use
NKP30 antibody was raised against recombinant extracellular domain(19-138aa) of human NKp30(NCR3) purified from E. coli.
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should titrate the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500-1:2000.
PBS, pH 7.4, 0.1% sodium azide
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Cytotoxicity-activating receptor that contributes to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis. Engagement of NCR3 by BAG6 also promotes dendritic cell (DC) maturation, both through killing those DCs that did not properly acquire a mature phenotype, and inducing NK cells to release TNFA and IFNG, which promotes DC maturation.
Human recombinant proteins, extracellular domain of NKp30(50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NKp30 (1:1000). Protein was visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Arrow indicates human recombinant protein NKp30 (residues 19~138).