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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NIX / BNIP3L Antibody (clone 2E11) LS‑C139429
BNIP3L antibody LS-C139429 is an unconjugated mouse monoclonal antibody to human BNIP3L (NIX). Validated for ELISA, IP and WB.
Catalog
Size
Price
LS-C139429-100
100 µg (1 mg/ml)
Unavailable

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Product Description

BNIP3L antibody LS-C139429 is an unconjugated mouse monoclonal antibody to human BNIP3L (NIX). Validated for ELISA, IP and WB.
About NIX / BNIP3L
Induces apoptosis. Interacts with viral and cellular anti-apoptosis proteins. Can overcome the suppressors BCL-2 and BCL-XL, although high levels of BCL-XL expression will inhibit apoptosis. Inhibits apoptosis induced by BNIP3. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates to mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. O60238 NM_004331 NP_004322.1

BNIP3L Antibody, NIP3-like protein X Antibody, NIP3L Antibody, NIX Antibody, BNIP3a Antibody, BNIP3H Antibody


Specifications

Target
Human NIX / BNIP3L
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [2E11]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • Western blot
  • Immunoprecipitation
  • ELISA
Immunogen
NIX / BNIP3L antibody was raised against bNIP3L (NP_004322, 43 a.a. ~ 131 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human BNIP3L
Usage
Sandwich ELISA: Recombinant protein. Western Blot: Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Immunoprecipitation

Immunoprecipitation of BNIP3L transfected lysate using anti-BNIP3L monoclonal antibody and Protein A Magnetic Bead.
Immunoprecipitation of BNIP3L transfected lysate using anti-BNIP3L monoclonal antibody and Protein A Magnetic Bead.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged BNIP3L is 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged BNIP3L is 0.3 ng/ml as a capture antibody.

Immunoprecipitation

Immunoprecipitation of BNIP3L transfected lysate using anti-BNIP3L monoclonal antibody and Protein A Magnetic Bead.
Immunoprecipitation of BNIP3L transfected lysate using anti-BNIP3L monoclonal antibody and Protein A Magnetic Bead.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged BNIP3L is 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged BNIP3L is 0.3 ng/ml as a capture antibody.

Immunoprecipitation

Immunoprecipitation of BNIP3L transfected lysate using anti-BNIP3L monoclonal antibody and Protein A Magnetic Bead.
Immunoprecipitation of BNIP3L transfected lysate using anti-BNIP3L monoclonal antibody and Protein A Magnetic Bead.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged BNIP3L is 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged BNIP3L is 0.3 ng/ml as a capture antibody.

Immunoprecipitation

Immunoprecipitation of BNIP3L transfected lysate using anti-BNIP3L monoclonal antibody and Protein A Magnetic Bead.
Immunoprecipitation of BNIP3L transfected lysate using anti-BNIP3L monoclonal antibody and Protein A Magnetic Bead.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged BNIP3L is 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged BNIP3L is 0.3 ng/ml as a capture antibody.


Requested From: 
Date Requested: 6/20/2018

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