Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(applications tested for the base form of this product only)
Specificity and Use
Nitrotyrosine antibody was raised against nitrated KLH.
Recognizes nitrated tyrosine residues.
Suitable for use in Western Blot and Immunohistochemistry. Western Blot: 1:100-1:1000 when tested against nitrated BSA and nitrated ovalbumin. Immunohistochemistry: 1:50-1:100 on paraffin embedded, 10% neutral buffer formalin fixed mouse liver (animal treated with Zymosan) and normal human cerebellum tissues. The tissues must be pretreated with heat induced epitope retrieval. (HIER). Incubation was for 10 minutes and the reaction was developed with HRP-DAB.
PBS, 0.1% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.