Synthetic Nitrotyrosine

Mouse

Synthetic (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

6B2-3G2

Unconjugated

Protein A purified

Unmodified

Protein-bound Nitrotyrosine.

Recognizes both free and protein-bound nitrotyrosine (Tyr-NO2), including human serum nitro albumin, nitro insulin and nitro haemoglobin. Nitrotyrosine arises from the nitration of protein tyrosine residues, by reactive nitrogen species such as peroxynitrite and nitrogen dioxide, and can be used as a measure of NO-dependent oxidative stress and cell damage. Increased levels of nitrotyrosine are detectable in various pathological conditions including atherosclerosis, rheumatoid arthritis, cardiovascular disease, lung cancer and vasculitis. The specificity of the antibody as determined by competition ELISA is: Nitrotyrosine:95 % of inhibition; Tyrosine:0 % of inhibition; Nitrotryptophan:4 % of inhibition; Aminotyrosine:0 % of inhibition; Phosphotyrosine:2 % of inhibition; Chlorotyrosine:3 % of inhibition;.

• Western blot (0.1 µg/ml)
• Immunoprecipitation (1 µg/ml)
• ELISA

PBS, 0.09% Sodium Azide

Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.