Human NIP3 / BNIP3

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2b Monoclonal

Unconjugated

Immunoaffinity purified

Unmodified

Recombinant fragment (Residues 1-163 of Human BNIP3). Epitope: The epitope recognized by the antibody resides within amino acids 112-124 of human BNIP3 molecule.

aa112-124

Species cross-reactivity: Cross-reacts with Human.

• IHC
• IHC - Paraffin (10 µg/ml)
• Western blot
• Immunoprecipitation
• ELISA

Immunohistochemistry: LS-B3305 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3305 was determined to be 10 ug/ml. Western Blot, Immunoprecipitation and Immunocytochemistry. Western Blot: 2-4 ug/ml. Detects a band at ~30kD and ~60kD. Positive control: Whole cell lysate from 293T cells transfected with BNIP3.

PBS, pH 7.4, 15 mM Sodium Azide

Store at 4°C while lyophilized. Once reconstituted, aliquot and store at -70°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q12983 NCBI: NM_004052 NP_004043.3