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Anti-NIFK / MKI67IP Antibody (aa140-189) IHC-plus™ LS-B1546

Note: This antibody replaces LS-C30455

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl - $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular NIFK / MKI67IP Antibodies

Anti-NIFK / MKI67IP Antibody (aa140-189) IHC-plus™ LS-B1546
Rabbit Polyclonal (IgG) to Human NIFK / MKI67IP
Human, Horse, Pig
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human NIFK / MKI67IP
Human, Horse, Pig
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human NIFK / MKI67IP
Rabbit
Human, Horse, Pig (tested or 100% immunogen sequence identity)
Monkey, Mouse, Rat, Dog, Rabbit (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with HRP, Biotin, FITC.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

NIFK / MKI67IP antibody was raised against synthetic peptide located between aa140-189 of human MKI67IP (Q9BYG3, NP_115766). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%); Marmoset, Rat, Panda, Dog, Rabbit (92%); Mouse, Zebra finch (91%); Galago, Hamster, Elephant, Bovine, Horse (85%); Bat (84%).
LS-E15066 - Lyophilized - 100 µg - $145.00
aa140-189
Human MKI67IP
Immunohistochemistry: LS-B1546 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1546 was determined to be 5 ug/ml.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About NIFK / MKI67IP

Q9BYG3 NM_032390 NP_115766.3

NIFK Antibody, HNIFK Antibody, MKI67IP Antibody, Nopp34 Antibody

NIFK / MKI67IP encodes a protein that interacts with the forkhead-associated domain of the Ki-67 antigen. The encoded protein may bind RNA and may play a role in mitosis and cell cycle progression. Multiple pseudogenes exist on chromosomes 5, 10, 12, 15, and 19.

Immunohistochemistry

Immunohistochemistry
Anti-MKI67IP antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1546 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
MKI67IP antibody LS-B1546 Western blot of Fetal Thymus lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Fetal Thymus Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/11/2016

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