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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NGFR / CD271 / TNR16 Antibody (aa202‑215) LS‑C154904
TNR16 antibody LS-C154904 is an unconjugated goat polyclonal antibody to TNR16 (NGFR / CD271) from human, rat, hamster and other species. Validated for Peptide-ELISA and WB.
Catalog
Size
Price
LS-C154904-100
100 µg (0.5 mg/ml)
Unavailable

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Product Description

TNR16 antibody LS-C154904 is an unconjugated goat polyclonal antibody to TNR16 (NGFR / CD271) from human, rat, hamster and other species. Validated for Peptide-ELISA and WB.
About NGFR / CD271 / TNR16
Plays a role in the regulation of the translocation of GLUT4 to the cell surface in adipocytes and skeletal muscle cells in response to insulin, probably by regulating RAB31 activity, and thereby contributes to the regulation of insulin-dependent glucose uptake (By similarity). Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. P08138 NM_002507 NP_002498.1

NGFR Antibody, CD271 Antibody, CD271 antigen Antibody, gp80-LNGFR Antibody, Nerve growth factor receptor Antibody, p75 ICD Antibody, p75(NTR) Antibody, p75NTR Antibody, TNFR superfamily, member 16 Antibody, NGF receptor Antibody, TNFRSF16 Antibody


Specifications

Target
Human NGFR / CD271 / TNR16
Host
Goat
Reactivity
Human, Rat, Hamster, Horse (tested or 100% immunogen sequence identity)
Predicted
Monkey, Bovine (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1 - 3 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:4000)
Immunogen
Synthetic peptide C-PEGSDSTAPSTQEP from an internal region of human NGFR (NP_002498.1). Percent identity by BLAST analysis: Human, Chimpanzee, Orangutan, Rat, Hamster, Horse (100%); Gorilla, Gibbon, Monkey, Marmoset, Elephant, Bovine (93%); Galago, Mouse, Panda, Rabbit, Porcine (86%).
Blocking Peptide
LS-E27767 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C154904. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa202-215
Specificity
Human NGFR. This antibody is expected to recognize the extracellular domain of p75NTR.
Usage
Peptide ELISA: antibody detection limit dilution 1:4000. Western blot: Approx 75kD band observed in Rat Heart lysates (calculated MW of 45.2kD according to Human NP_002498.1 and 45.4kD according to Rat NP_036742.2. The observed molecular weight corresponds to earlier findings in literature with different antibodies (Nykjaer et al, Curr Opin Neurobiol. 2005 Feb;15(1):49-57). Recommended concentration: 1-3 ug/ml.
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

NGFR antibody (1 ug/ml) staining of Rat Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
NGFR antibody (1 ug/ml) staining of Rat Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

NGFR antibody (1 ug/ml) staining of Rat Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
NGFR antibody (1 ug/ml) staining of Rat Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

NGFR antibody (1 ug/ml) staining of Rat Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
NGFR antibody (1 ug/ml) staining of Rat Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

NGFR antibody (1 ug/ml) staining of Rat Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
NGFR antibody (1 ug/ml) staining of Rat Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.


Requested From: 
Date Requested: 6/21/2018

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