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Anti-NGF Antibody (aa84-115) LS-C96702

Ordering

Wt. Vol. Conc. Price
- 400 µl - Unavailable

Most Popular NGF Antibodies

Anti-NGF Antibody LS-C104703
Rabbit Polyclonal to Human NGF
Human
IHC, Western blot, ELISA, Neutralization
Unconjugated
Immunohistochemistry Image
Anti-NGF Antibody (Biotin) LS-C104735
Rabbit Polyclonal to Human NGF
Human
IHC, Western blot, ELISA, Neutralization
Biotin Conjugated
Immunohistochemistry Image
Anti-NGF Antibody (N-Terminus) LS-C171793
Rabbit Polyclonal to Human NGF
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human NGF
Human
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated

Details

Human NGF
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Ammonium sulfate precipitation
Unmodified

Applications

  • IHC - Paraffin (1:10 - 1:50)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)

  • IHC - Paraffin

Specificity and Use

LS-E9250 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C96702. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa84-115
This NGFB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 84-115 amino acids from the Central region of human NGFB.

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About NGF

P01138 NM_002506 NP_002497.2

NGF Antibody, Beta-nerve growth factor Antibody, ProNGF Antibody, Beta-NGF Antibody, HSAN5 Antibody, NGFB Antibody

This gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons.

Immunohistochemistry

Immunohistochemistry
Formalin-fixed and paraffin-embedded human brain tissue reacted with NGFB antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Immunofluorescence

Immunofluorescence
Confocal immunofluorescence of NGFB Antibody with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot

Western blot
Western blot of NGFB Antibody in NCI-H460 cell line lysates (35 ug/lane). NGFB (arrow) was detected using the purified antibody.

Flow Cytometry

Flow Cytometry
NGFB Antibody flow cytometry of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Requested From: 
Date Requested: 3/27/2017

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