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NFYB

nuclear transcription factor Y, beta

NFYB is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants.

Gene Name: nuclear transcription factor Y, beta
Synonyms: NFYB, CBF-A, CBF-B, HAP3, NF-YB
Target Sequences: NM_006166 NP_006157.1 P25208

☰ Filters
Antibodies (31)
Proteins (5)
ELISA Kits (2)
Cell-Based (1)
DNA Binding (1)
Over-Expression Lysate (2)
Primary (31)
Recombinant (3)
NFYB (38)
Human (33)
Mouse (19)
Rat (14)
Bat (3)
Bovine (4)
Chicken (7)
Chimpanzee (6)
Dog (7)
Gibbon (6)
Guinea pig (6)
Horse (3)
Monkey (6)
Pig (7)
Rabbit (6)
IHC (6)
IHC-P (3)
WB (30)
ELISA (14)
GS (2)
IF (4)
IP (2)
Peptide-ELISA (2)
RNAi (1)
rabbit (29)
mouse (2)
Product Group
IHCPlus (1)
IgG (19)
IgG1 (1)
IgG2a,k (1)
monoclonal mc (2)
polyclonal pc (29)
6H6 (1)
12 x 8-Well Microstrips (1)
96-Well Microplate (1)
APC Conjugated (1)
Azide-free (1)
Biotin Conjugated (3)
FITC Conjugated (3)
HRP Conjugated (3)
PE Conjugated (1)
Unconjugated (20)
N-Terminus (9)
aa17-46 (7)
C-Terminus (4)
10-90 aa, N-terminal (1)
Gln30 (1)
aa1-50 (1)
No (38)
Adherent Cell Cultures (1)
Cell Lysates (1)
Nuclear Lysates (1)
GST (1)
GST, N-terminus (1)
Myc-DDK (Flag) (3)
Human (4)
293T Cells (1)
E. coli (1)
HEK 293 Cells (2)
Wheat Germ Extract (1)
Purified (2)
NFYB Antibody - Anti-NFYB antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
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NFYB Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Human
ELISA, GS, IHC, IHC-P, WB
Unconjugated
50 µl/$515
NFYB Antibody - Immunohistochemical analysis of NFYB staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected with HRP and DAB as chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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NFYB Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Chicken, Bovine, Mouse, Dog, Rat, Pig, Human
IHC, IHC-P, WB
Unconjugated
30 µl/$255; 100 µl/$299; 200 µl/$351
NFYB Antibody - Transfected 293T Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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NFYB Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Human
WB
Unconjugated
100 µl/$424
NFYB Antibody - Immunofluorescence analysis of MCF7 cells.
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NFYB Rabbit anti-Human Polyclonal Antibody
Mouse, Human
IF, IHC, WB
Unconjugated
20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503
NFYB Antibody - Western Blot analysis of NFYB expression in transfected 293T cell line by NFYB monoclonal antibody (M01), clone 6H6.Lane 1: NFYB transfected lysate(22.8 KDa).Lane 2: Non-transfected lysate.
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NFYB Mouse anti-Human Monoclonal (6H6) Antibody
Human
ELISA, RNAi, WB
Unconjugated
100 µg/$423
NFYB Antibody - Western blot of NF-YB antibody
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NFYB Rabbit anti-Human Polyclonal (10-90 aa, N-terminal) Antibody
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
50 µg/$295; 100 µg/$335; 200 µg/$394
NFYB Antibody - Immunohistochemistry of paraffin-embedded human small intestine at dilution 1:100
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NFYB Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC, IHC-P
Unconjugated
50 µl/$294; 100 µl/$360
NFYB Antibody - Staining 293 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
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NFYB Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
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NFYB Rabbit anti-Human Polyclonal Antibody
Mouse, Human
IF, WB
Unconjugated
60 µl/$331; 120 µl/$403; 200 µl/$529
Select
NFYB Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Human
ELISA, GS, WB
Unconjugated
100 µg/$567
Select
NFYB Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Mouse, Human
ELISA, IP, WB
Unconjugated
100 µl/$917
Select
NFYB Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Mouse, Human
ELISA, IP, WB
Unconjugated
100 µg/$973
Select
NFYB Rabbit anti-Human Polyclonal (Gln30) Antibody
Mouse, Rat, Human
IHC, WB
Unconjugated
50 µl/$321; 100 µl/$403
NFYB Antibody - Fluorescent confocal image of HeLa cell stained with NFYB Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NFYB primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NFYB immunoreactivity is localized to nucleus significantly.
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NFYB Rabbit anti-Human Polyclonal (aa17-46) Antibody
Human
IF, WB
Unconjugated
400 µl/$393
Select
NFYB Rabbit anti-Human Polyclonal (aa17-46) Antibody
Human
ELISA, WB
Unconjugated
200 µl/$734
Select
NFYB Rabbit anti-Human Polyclonal (aa17-46) (Biotin) Antibody
Human
ELISA, WB
Biotin Conjugated
200 µl/$952
Select
NFYB Rabbit anti-Human Polyclonal (aa17-46) (FITC) Antibody
Human
ELISA, WB
FITC Conjugated
200 µl/$952
Select
NFYB Rabbit anti-Human Polyclonal (aa17-46) (APC) Antibody
Human
ELISA, WB
APC Conjugated
200 µl/$952
Select
NFYB Rabbit anti-Human Polyclonal (aa17-46) (Azide-free) (HRP) Antibody
Human
ELISA, WB
HRP Conjugated, Azide-free
200 µl/$952
Select
NFYB Rabbit anti-Human Polyclonal (aa17-46) (PE) Antibody
Human
ELISA, WB
PE Conjugated
200 µl/$952
NFYB Antibody - Western blot analysis of extracts from 293 cells, using NFYB antibody.
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NFYB Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ELISA, WB
Unconjugated
100 µg/$357
NFYB Antibody - Western blot analysis of lysates from 293 cells, using NFYB Antibody. The lane on the right is blocked with the synthesized peptide.
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NFYB Rabbit anti-Human Polyclonal (aa1-50) Antibody
Mouse, Rat, Human
Peptide-ELISA, WB
Unconjugated
50 µl/$334; 100 µl/$397
Select
NFYB Mouse anti-Human Monoclonal Antibody
Human
WB
Unconjugated
100 µg/$786
NFYB Antibody - NFYB antibody Western blot of Transfected 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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NFYB Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Human
WB
Unconjugated
100 µl/$424
NFYB Antibody - All lanes: Anti-NFYB Antibody at 1:1000 dilution. Lane 1: A431 whole cell lysate. Lane 2: HeLa whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 23 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
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NFYB Rabbit anti-Human Polyclonal Antibody
Mouse, Human
WB
Unconjugated
100 µl/$386
Viewing 1-25 of 38 product results



If you do not find the reagent or information you require, please contact Customer.Support@LSBio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).