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Anti-NFYB Antibody (N-Terminus) LS-C18766

Note: This antibody replaces LS-C9330

Ordering

Wt. Vol. Conc. Price
100 µg - - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular NFYB Antibodies

Anti-NFYB Antibody (N-Terminus) IHC-plus™ LS-B718
Rabbit Polyclonal to Human NFYB
Human
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image
Anti-NFYB Antibody (N-Terminus) LS-C42717
Rabbit Polyclonal (IgG) to Human NFYB
Human, Mouse
Western blot, Immunoprecipitation, ELISA, Gel shift
Unconjugated
Anti-NFYB Antibody (N-Terminus) LS-C131044
Rabbit Polyclonal to Human NFYB
Human
ELISA
Unconjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human NFYB
Human
Western blot, Immunoprecipitation, ELISA
Unconjugated

Details

Human NFYB
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Ion exchange chromatography
Unmodified

Applications

  • Western blot (1:500)
  • Immunoprecipitation
  • ELISA

Specificity and Use

N-Terminus
NF-Y (B subunit) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Suitable for immunoprecipitation, immunoblotting, ELISA and supershift assays. This product was assayed by immunoblot and found to be reactive against the 25 kD B subunit of NF-Y at a dilution of 1:500 followed by reaction with Peroxidase conjugated Affinity Purified anti- Rabbit IgG [H&L] (Goat) catalog no. LS-C60884. Anti-NF-Y (13 subunit specific) is suitable for the detection by immunoblot of human and mouse NF-Y (13 subunit specific). Minimal reaction was observed by immunoblot against the 35 kD A subunit of NF-Y. This product was also tested in a gel supershift assay and found to be reactive against human and mouse NF-Y using 2.0 to 4.0 ul per assay. This product was assayed against NF-Y B subunit peptide in an antibody capture ELISA using (#LS-C60884) Peroxidase conjugated Affinity Purified anti- Rabbit IgG [H&L] (Goat) and ABTS). A dilution of 1:4000 is suggested from this experiment. Minimal reactivity was observed by ELISA against the A subunit of NF-Y.

Packaging

0.01 M sodium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Store vial at -20 C or below prior to opening. Dilute only prior to immediate use. Aliquot contents and freeze at -20 C or below. Avoid cycles of freezing and thawing.
For research use only.

About NFYB

P25208 NM_006166 NP_006157.1

NFYB Antibody, CBF-A Antibody, CBF-B Antibody, HAP3 Antibody, NF-YB Antibody

NFYB is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif.

Requested From: United States
Date Requested: 12/7/2016

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