Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
NFYB is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif.
Western Blot analysis of NFYB expression in transfected 293T cell line by NFYB monoclonal antibody (M01), clone 6H6.Lane 1: NFYB transfected lysate(22.8 KDa).Lane 2: Non-transfected lysate.
Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged NFYB is approximately 0.1 ng/ml as a capture antibody.
Western blot analysis of NFYB over-expressed 293 cell line, cotransfected with NFYB Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with NFYB monoclonal antibody (M01), clone 6H6 . GAPDH ( 36.1 kDa ) used as specificity and loading control.
Requested From: United States
Date Requested: 2/22/2017