(tested or 100% immunogen sequence identity)
Ion exchange chromatography
- Western blot (1:1000)
- ELISA (1:10000)
- Gel shift
NFYA antibody was raised against nF-Y (A subunit) peptide corresponding to a region near the N-terminus conjugated to KLH.
Recognizes both differentially spliced 35kD and 40kD forms of the A subunit of NF-Y. No reactivity against the 25kD B subunit. Species cross-reactivity: Human, mouse.
ELISA: 1:10000. Western Blot: 1:1000. Gel supershift assays: 2 ul per test.
PBS, 0.01% Sodium Azide
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.