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Anti-NFKBIA / IKB Alpha / IKBA Antibody (Ser32) IHC-plus™ LS-B1573

Note: This antibody replaces LS-C34346
Catalog Size Price
LS-B1573-50 50 µg (1 mg/ml) Unavailable

Most Popular NFKBIA / IKB Alpha / IKBA Antibodies

Anti-NFKBIA / IKB Alpha / IKBA Antibody (C-Terminus) IHC-plus™ LS-B717
Rabbit Polyclonal to Human NFKBIA / IKB Alpha / IKBA
Human, Mouse, Rat
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image
Anti-NFKBIA / IKB Alpha / IKBA Antibody LS-C48310
Mouse Monoclonal (IgG1) to Human NFKBIA / IKB Alpha / IKBA
Human, Mouse
IHC, Western blot, Immunoprecipitation
Unconjugated
Western blot Image
Anti-NFKBIA / IKB Alpha / IKBA Antibody LS-C123817
Rabbit Polyclonal (IgG) to Human NFKBIA / IKB Alpha / IKBA
Human, Mouse, Rat
IHC, Western blot
Unconjugated
Anti-NFKBIA / IKB Alpha / IKBA Antibody (aa12-41) LS-C164850
Rabbit Polyclonal to Human NFKBIA / IKB Alpha / IKBA
Human
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-NFKBIA / IKB Alpha / IKBA Antibody (phospho-Ser32/Ser36) LS-C352580
Rabbit Polyclonal to Human NFKBIA / IKB Alpha / IKBA
Human, Mouse, Rat, Bovine, Pig, Sheep
IHC - Paraffin, ICC, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed
Rabbit Polyclonal (IgG) to Human NFKBIA / IKB Alpha / IKBA
Human, Mouse, Rat
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated

Details

Human NFKBIA / IKB Alpha / IKBA
Rabbit
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (20 µg/ml)
  • Immunofluorescence
  • Western blot

Specificity and Use

Synthetic peptide from human NFKBIA.
Ser32
Amino acids surrounding Ser 32 of human NFKBIA
Immunohistochemistry: LS-B1573 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1573 was determined to be 20 ug/ml.

Packaging

PBS, 0.02% sodium azide, 50% glycerol.
Long term: -70°C; Short term: -20°C
For research use only.

About NFKBIA / IKB Alpha / IKBA

P25963 NM_020529 NP_065390.1

NFKBIA Antibody, IkB-alpha Antibody, IKBA Antibody, IkappaBalpha Antibody, MAD3 Antibody, NF-kappa-B inhibitor alpha Antibody, NFKBI Antibody, I-kappa-B-alpha Antibody, IKB Alpha Antibody, MAD-3 Antibody

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.

Immunohistochemistry

Immunohistochemistry
Anti-IKB Alpha antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1573 concentration 20 ug/ml.

Immunofluorescence

Immunofluorescence
Immunofluorescence staining of methanol-fixed HeLa cells using IkB-a(Ab-32/36) antibody.

Western blot

Western blot
Western blot of extracts from 293 and 3T3 cells using IkB-a(Ab-32/36) antibody.

Requested From: 
Date Requested: 10/17/2017

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