Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52.
Detection of Human NFkappa B2 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line untreated (-) or treated (+) with 100 ng/ml Recombinant Human CD40 Ligand/TNFSF5 aa 108-261 for 4 hours. Gels were loaded with 20 ug of cytoplasmic (Cyto) and 10 ug of nuclear extracts (Nuc). For additional reference, lysates of Raji human Burkitt's lymphoma cell line were included. PVDF membrane was probed with 0.5 ug/ml Mouse Anti-Human NF kappa B2 Monoclonal Antibody followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody. Specific bands for NFkappa B2 were detected at approximately 52 kD and 100 kD (as indicated). This experiment was conducted under reducing conditions.
Detection of NF kappa B2-regulated Genes by Chromatin Immunoprecipitation. Jurkat human acute T cell leukemia cell line treated with 50 ng/ml PMA and 200 ng/ml calcium ionomycin overnight was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. NF kappa B2/DNA complexes were immunoprecipitated using 5 ug Mouse Anti-Human NF kappa B2 Monoclonal Antibody or control antibody for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Mouse IgG Secondary Antibody. Immune complexes were captured using 50 ul of MagCellect Streptavidin Ferrofluid and DNA was purified using chelating resin solution. The c-myc promoter was detected by standard PCR.