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Anti-NFKB2 Antibody (aa1-447, clone 291319) LS-C150014


Wt. Vol. Conc. Price
100 µg - - $315
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular NFKB2 Antibodies

Anti-NFKB2 Antibody (aa711-758) LS-C29758
Rabbit Polyclonal (IgG) to Human NFKB2
Human, Bovine, Horse
IHC - Paraffin, Western blot
Immunohistochemistry Image
Anti-NFKB2 Antibody (aa1-19) IHC-plus™ LS-B396
Rabbit Polyclonal to Human NFKB2
IHC - Paraffin, Western blot, ELISA
Immunohistochemistry Image
Anti-NFKB2 Antibody LS-C62123
Mouse Monoclonal (IgG2b,k) to Human NFKB2
Human, Mouse, Rat
Western blot, ELISA
Western blot Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 291319] (IgG1) to Human NFKB2
ICC, Western blot, Immunoprecipitation


Human NFKB2
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [291319]
Protein A/G purified


  • ICC (8 - 25 µg/ml)
  • Western blot (0.5 µg/ml)
  • Immunoprecipitation

Specificity and Use

NFKB2 antibody was raised against e. coli-derived recombinant human NF kappa B2 Met1-Asn447 Accession # Q00653.
Detects human NFkB2 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with endogenous mouse NFkB2 in NIH 3T3.
Western Blot, 0.5 µg/ml, Chromatin Immunoprecipitation (ChIP), 5 ug/5 x 106 cells. Immunocytochemistry, 8-25 µg/ml, Immersion fixed human peripheral blood mononuclear cells.


Lyophilized from 0.2 um filtered solution in PBS, trehalose
Sterile PBS. Resuspend to 200 ul
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About NFKB2

Q00653 NM_002502 NP_002493.3

NFKB2 Antibody, DNA-binding factor KBF2 Antibody, LYT10 Antibody, Oncogene Lyt-10 Antibody, p52 Antibody, LYT-10 Antibody, NF-kB2 Antibody, p105 Antibody, H2TF1 Antibody

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52.

Western blot

Western blot
Detection of Human NFkappa B2 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line untreated (-) or treated (+) with 100 ng/ml Recombinant Human CD40 Ligand/TNFSF5 aa 108-261 for 4 hours. Gels were loaded with 20 ug of cytoplasmic (Cyto) and 10 ug of nuclear extracts (Nuc). For additional reference, lysates of Raji human Burkitt's lymphoma cell line were included. PVDF membrane was probed with 0.5 ug/ml Mouse Anti-Human NF kappa B2 Monoclonal Antibody followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody. Specific bands for NFkappa B2 were detected at approximately 52 kD and 100 kD (as indicated). This experiment was conducted under reducing conditions.

Chromatin Immunoprecipitation

Chromatin Immunoprecipitation
Detection of NF kappa B2-regulated Genes by Chromatin Immunoprecipitation. Jurkat human acute T cell leukemia cell line treated with 50 ng/ml PMA and 200 ng/ml calcium ionomycin overnight was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. NF kappa B2/DNA complexes were immunoprecipitated using 5 ug Mouse Anti-Human NF kappa B2 Monoclonal Antibody or control antibody for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Mouse IgG Secondary Antibody. Immune complexes were captured using 50 ul of MagCellect Streptavidin Ferrofluid and DNA was purified using chelating resin solution. The c-myc promoter was detected by standard PCR.

Requested From: United States
Date Requested: 10/27/2016

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