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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-B716-50 50 µl (85 mg/ml) $485 
NFKB1 / NF-Kappa-B Antibody - Anti-NFKB1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
NFKB1 / NF-Kappa-B Antibody - Anti-NFKB p50 (NFKB1) Antibody - Western Blot. All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a:1:1000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5 each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2000 for 1 h. Washes with TBS preceded color development.
NFKB1 / NF-Kappa-B Antibody - Immunoblot of HeLa cell extract using a 1:1000 dilution of anti-NFKB1.
NFKB1 / NF-Kappa-B Antibody - Anti-NFKB1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
NFKB1 / NF-Kappa-B Antibody - Anti-NFKB p50 (NFKB1) Antibody - Western Blot. All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a:1:1000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5 each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2000 for 1 h. Washes with TBS preceded color development.
NFKB1 / NF-Kappa-B Antibody - Immunoblot of HeLa cell extract using a 1:1000 dilution of anti-NFKB1.
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IHCPlus™ NFKB1 / NF‑Kappa‑B Antibody N‑Terminus Polyclonal ELISA,GS,IF,IHC,IP,WB LS‑B716

IHCPlus™ NFKB1 / NF‑Kappa‑B Antibody N‑Terminus Polyclonal ELISA,GS,IF,IHC,IP,WB LS‑B716

Note: This antibody replaces LS-C18853, LS-C42711, LS-C37633
Antibody:
Rabbit Polyclonal to Human NFKB1 / NF-Kappa-B
Application:
IHC, IHC-P, IF, WB, IP, ELISA, GS
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485 
LS-B716-50
50 µl
85 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human NFKB1 / NF-Kappa-B
Application:
IHC, IHC-P, IF, WB, IP, ELISA, GS
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
NF-Kappa-B antibody LS-B716 is an unconjugated rabbit polyclonal antibody to human NF-Kappa-B (NFKB1 ). Validated for ELISA, GS, IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human NFKB1 / NF-Kappa-B
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Delipidated and defibrinated
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:500)
  • Immunofluorescence
  • Western blot (1:1000)
  • Immunoprecipitation
  • ELISA
  • Gel shift
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Epitope
N-Terminus
Specificity
Human NFkB p50 (NFKB1) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Usage
Immunohistochemistry: LS-B716 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B716 was determined to be 1:500.
Presentation
0.01% Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NFKB1 / NF-Kappa-B
P19838 NM_003998 NP_003989.2

Validation

NFKB1 / NF-Kappa-B Antibody - Anti-NFKB1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
Anti-NFKB1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
Anti-NFKB1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.

Publications (1)

Nuclear factor-B contributes to neuron-dependent induction of glutamate transporter-1 expression in astrocytes. Ghosh M, Yang Y, Rothstein JD, Robinson MB. The Journal of neuroscience : the official journal of the Society for Neuroscience. 2011 31:9159-69.

Customer Reviews (0)


Requested From: United States
Date Requested: 12/6/2019