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| Catalog Number | Size | Price |
|---|---|---|
| LS-B716-50 | 50 µl (85 mg/ml) | $515 |
![NFKB1 / NF-Kappa-B Antibody - Anti-NFKB p50 (NFKB1) Antibody - Western Blot. All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a:1:1000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5 each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2000 for 1 h. Washes with TBS preceded color development.](https://lsbio-7d62.kxcdn.com/image2/ihc-plus-nfkb1-nf-kappa-b-antibody-n-terminus-ls-b716/62544_27851.jpg)
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IHC‑plus™ Polyclonal Rabbit anti‑Human NFKB1 / NF‑Kappa‑B Antibody (N‑Terminus, IHC, WB) LS‑B716
IHC‑plus™ Polyclonal Rabbit anti‑Human NFKB1 / NF‑Kappa‑B Antibody (N‑Terminus, IHC, WB) LS‑B716
Note: This antibody replaces LS-C18853, LS-C42711, LS-C37633
Antibody:
NFKB1 / NF-Kappa-B Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, IHC-P, WB, ELISA, GS
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
NFKB1 / NF-Kappa-B Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, IHC-P, WB, ELISA, GS
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
NF-Kappa-B antibody LS-B716 is an unconjugated rabbit polyclonal antibody to human NF-Kappa-B (NFKB1) (N-Terminus). Validated for ELISA, GS, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human NFKB1 / NF-Kappa-B
Synonyms
NFKB1 | Ebp- 1 | EBP-1 | NF-KappaB p50 | NFkappaB | NFKB-p50 | KBF1 | NF-kappa-B | NF-kappaB | NF-kB1 | NFKB-p105 | p105 | p50 | DNA binding factor KBF1 | DNA-binding factor KBF1 | NF-kappabeta
Host
Rabbit
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Delipidated and defibrinated
Modifications
Unmodified
Immunogen
Human NFkB p50 (NFKB1) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Epitope
N-Terminus
Applications
- IHC
- IHC - Paraffin (1:500)
- Western blot (1:500 - 1:2000)
- ELISA (1:5000 - 1:25000)
- Gel shift
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B716 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B716 was determined to be 1:500.
Presentation
Antiserum, 0.01% Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About NFKB1 / NF-Kappa-B
Validation
Anti-NFKB1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
Anti-NFKB1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
See More About...
LSBio Ratings
IHC-plus™ NFKB1 / NF-Kappa-B Antibody (N-Terminus) for IHC, WB/Western, ELISA LS-B716 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (1)
Nuclear factor-B contributes to neuron-dependent induction of glutamate transporter-1 expression in astrocytes. Ghosh M, Yang Y, Rothstein JD, Robinson MB. The Journal of neuroscience : the official journal of the Society for Neuroscience. 2011 31:9159-69.
Customer Reviews (0)
Featured Products
Species:
Human, Chicken
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot
![NFKB1 / NF-Kappa-B Antibody - Immunoperoxidase of monoclonal antibody to NFKB1 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml]](https://lsbio-7d62.kxcdn.com//image2/nfkb1-nf-kappa-b-antibody-clone-3f6-ls-c133346/51309_1017279.jpg)
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/18/2024
Date Requested: 4/18/2024
