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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-B1668-50 50 µg (0.5 mg/ml) $425 
NFKB1 / NF-Kappa-B Antibody - Anti-NFKB1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 15 ug/ml.
NFKB1 / NF-Kappa-B Antibody - MOLT4 cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with mouse anti-NF-kappaB p50 antibody (clone 4D1). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
NFKB1 / NF-Kappa-B Antibody - Anti-NFKB1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 15 ug/ml.
NFKB1 / NF-Kappa-B Antibody - MOLT4 cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with mouse anti-NF-kappaB p50 antibody (clone 4D1). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
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IHCPlus™ NFKB1 / NF‑Kappa‑B Antibody Monoclonal IF,IHC,WB LS‑B1668

IHCPlus™ NFKB1 / NF‑Kappa‑B Antibody Monoclonal IF,IHC,WB LS‑B1668

Note: This antibody replaces LS-C40935
Antibody:
Mouse Monoclonal to Human NFKB1 / NF-Kappa-B
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B1668-50
50 µg
0.5 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human NFKB1 / NF-Kappa-B
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
NF-Kappa-B antibody LS-B1668 is an unconjugated mouse monoclonal antibody to human NF-Kappa-B (NFKB1 ). Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 4 publications.
Target
Human NFKB1 / NF-Kappa-B
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
4D1
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (15 µg/ml)
  • Immunofluorescence
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Recombinant human NFKB1.
Specificity
Recombinant full-length human NF-kB p50
Usage
Immunohistochemistry: LS-B1668 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1668 was determined to be 15 ug/ml.
Presentation
Phosphate-buffered solution, pH 7.2, 0.09% sodium azide.
Storage
Store at 4°C. Avoid freezing.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NFKB1 / NF-Kappa-B
P19838 NM_003998 NP_003989.2

Validation

NFKB1 / NF-Kappa-B Antibody - Anti-NFKB1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 15 ug/ml.
Anti-NFKB1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 15 ug/ml.
Anti-NFKB1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 15 ug/ml.

Publications (4)

Requirement for both JAK-mediated PI3K signaling and ACT1/TRAF6/TAK1-dependent NF-kappaB activation by IL-17A in enhancing cytokine expression in human airway epithelial cells. Huang F, Kao CY, Wachi S, Thai P, Ryu J, Wu R. Journal of immunology (Baltimore, Md. : 1950). 2007 179:6504-13.

Requirements for two proximal NF-kappaB binding sites and IkappaB-zeta in IL-17A-induced human beta-defensin 2 expression by conducting airway epithelium. Kao CY, Kim C, Huang F, Wu R. The Journal of biological chemistry. 2008 283:15309-18.

NF-B regulates MICA gene transcription in endothelial cell through a genetically inhibitable control site. Lin D, Lavender H, Soilleux EJ, O'Callaghan CA. The Journal of biological chemistry. 2012 287:4299-310.

1,25-dihydroxyvitamin D3 impairs NF- kappa B activation in human naïve B cells. Geldmeyer-Hilt K, Heine G, Hartmann B, Baumgrass R, Radbruch A, Worm M. Biochemical and biophysical research communications. 2011 Apr;407:699-702.

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Requested From: United States
Date Requested: 12/13/2019