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Anti-NFKB1 / NF-Kappa-B Antibody (clone 4D1) IHC-plus™ LS-B1668

Note: This antibody replaces LS-C40935


Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular NFKB1 / NF-Kappa-B Antibodies

Anti-NFKB1 / NF-Kappa-B Antibody (C-Terminus) LS-C112312
Rabbit Polyclonal to Human NFKB1 / NF-Kappa-B
Human, Monkey, Mouse, Rat, Dog, Horse, Rabbit
Western blot
Western blot Image
Anti-NFKB1 / NF-Kappa-B Antibody (N-Terminus) IHC-plus™ LS-B8072
Rabbit Polyclonal (IgG) to Human NFKB1 / NF-Kappa-B
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Zebrafish
IHC - Paraffin, Western blot
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed Publications Publications (4)
Mouse Monoclonal [clone 4D1] (IgG1) to Human NFKB1 / NF-Kappa-B
IHC - Paraffin, Immunofluorescence, Western blot


Human NFKB1 / NF-Kappa-B
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [4D1]
Affinity purified


  • IHC - Paraffin (15 µg/ml)
  • Immunofluorescence
  • Western blot

Specificity and Use

NFKB1 / NF-Kappa-B antibody was raised against recombinant human NFKB1.
Recombinant full-length human NF-kB p50
Immunohistochemistry: LS-B1668 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1668 was determined to be 15 ug/ml.


Phosphate-buffered solution, pH 7.2, 0.09% sodium azide.
+4°C, avoid freezing
For research use only.

Publications (4)

Requirement for both JAK-mediated PI3K signaling and ACT1/TRAF6/TAK1-dependent NF-kappaB activation by IL-17A in enhancing cytokine expression in human airway epithelial cells. Huang F, Kao CY, Wachi S, Thai P, Ryu J, Wu R. Journal of immunology (Baltimore, Md. : 1950). 2007 179:6504-13. [PubMed:17982039]
Requirements for two proximal NF-kappaB binding sites and IkappaB-zeta in IL-17A-induced human beta-defensin 2 expression by conducting airway epithelium. Kao CY, Kim C, Huang F, Wu R. The Journal of biological chemistry. 2008 283:15309-18. [PubMed:18362142] [PMC:PMC2397472]
NF-B regulates MICA gene transcription in endothelial cell through a genetically inhibitable control site. Lin D, Lavender H, Soilleux EJ, O'Callaghan CA. The Journal of biological chemistry. 2012 287:4299-310. [PubMed:22170063] [PMC:PMC3281736]
1,25-dihydroxyvitamin D3 impairs NF- kappa B activation in human naïve B cells. Geldmeyer-Hilt K, Heine G, Hartmann B, Baumgrass R, Radbruch A, Worm M. Biochemical and biophysical research communications. 2011 Apr;407:699-702. [Full Text Article] [PubMed:21420936]

About NFKB1 / NF-Kappa-B

P19838 NM_003998 NP_003989.2

NFKB1 Antibody, Ebp- 1 Antibody, EBP-1 Antibody, NF-KappaB p50 Antibody, NFkappaB Antibody, NFKB-p50 Antibody, KBF1 Antibody, NF-kappa-B Antibody, NF-kappaB Antibody, NF-kB1 Antibody, NFKB-p105 Antibody, p105 Antibody, p50 Antibody, DNA binding factor KBF1 Antibody, DNA-binding factor KBF1 Antibody, NF-kappabeta Antibody

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis.


Anti-NFKB1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1668 concentration 15 ug/ml.

Western blot

Western blot
MOLT4 cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with mouse anti-NF-kappaB p50 antibody (clone 4D1). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.

Requested From: United States
Date Requested: 10/24/2016

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