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Antibodies > NFIA / Nuclear Factor 1 > LS-B9077
 
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Catalog Number Size Price
LS-B9077-200 200 µl $460 
NFIA / Nuclear Factor 1 Antibody - Anti-NFIA / Nuclear Factor 1 antibody IHC staining of human skin, epidermis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
NFIA / Nuclear Factor 1 Antibody - Fluorescent confocal image of HeLa cell stained with NFIA Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NFIA primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NFIA immunoreactivity is localized to Nucleus significantly.
NFIA / Nuclear Factor 1 Antibody - NFIA Antibody western blot of HepG2 cell line lysates (35 ug/lane). The NFIA antibody detected the NFIA protein (arrow).
NFIA / Nuclear Factor 1 Antibody - Anti-NFIA / Nuclear Factor 1 antibody IHC staining of human skin, epidermis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
NFIA / Nuclear Factor 1 Antibody - Fluorescent confocal image of HeLa cell stained with NFIA Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NFIA primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NFIA immunoreactivity is localized to Nucleus significantly.
NFIA / Nuclear Factor 1 Antibody - NFIA Antibody western blot of HepG2 cell line lysates (35 ug/lane). The NFIA antibody detected the NFIA protein (arrow).
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IHC‑plus™ Polyclonal Rabbit anti‑Human NFIA / Nuclear Factor 1 Antibody (aa470‑498, IHC, IF, WB) LS‑B9077

IHC‑plus™ Polyclonal Rabbit anti‑Human NFIA / Nuclear Factor 1 Antibody (aa470‑498, IHC, IF, WB) LS‑B9077

IHC Validated
(4.0) Rating
Submit Review LSBio Guarantee
Note: This antibody replaces LS-C164846
Antibody:
NFIA / Nuclear Factor 1 Rabbit anti-Human Polyclonal (aa470-498) Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B9077-200
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
NFIA / Nuclear Factor 1 Rabbit anti-Human Polyclonal (aa470-498) Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Nuclear Factor 1 antibody LS-B9077 is an unconjugated rabbit polyclonal antibody to human Nuclear Factor 1 (NFIA) (aa470-498). Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human NFIA / Nuclear Factor 1
Synonyms
NFIA | CTF | NF1-A | NF-I/A | NFI-L | Nuclear Factor 1 | TGGCA-binding protein | NFI-A | Nuclear factor 1 A-type | KIAA1439 | Nuclear factor 1/A | Nuclear factor I/A
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Chicken (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Epitope
aa470-498
Specificity
This NFIA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 470-498 amino acids from the C-terminal region of human NFIA.
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NFIA / Nuclear Factor 1
Q12857 NM_005595 NP_005586.1

Validation

NFIA / Nuclear Factor 1 Antibody - Anti-NFIA / Nuclear Factor 1 antibody IHC staining of human skin, epidermis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NFIA / Nuclear Factor 1 antibody IHC staining of human skin, epidermis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NFIA / Nuclear Factor 1 antibody IHC staining of human skin, epidermis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
See More About...
About LSBio Validation process

LSBio Ratings

IHC-plus™ NFIA / Nuclear Factor 1 Antibody (aa470-498) for IHC, IF/Immunofluorescence, WB/Western LS-B9077 has an LSBio Rating of
(4.0) Rating
Laboratory Validation Score (4)

Publications (0)

Submit a Publication for LS-B9077

Customer Reviews (0)

Submit a review for LS-B9077

Featured Products

NFIA / Nuclear Factor 1 Antibody - Immunohistochemical analysis of NFIA staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
NFIA / Nuclear Factor 1 Antibody (N‑Terminus) LS‑C354251
Species: Human, Mouse, Rat, Bovine, Pig, Chicken, Zebrafish
Applications: IHC, IHC - Paraffin, Western blot
NFIA / Nuclear Factor 1 Antibody - Anti-NFIA antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
NFIA / Nuclear Factor 1 Antibody (aa348‑397) LS‑B1549
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot
NFIA / Nuclear Factor 1 Antibody - Immunohistochemistry - Anti-NFIA Picoband Antibody
NFIA / Nuclear Factor 1 Antibody LS‑C661861
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Western blot, Flow Cytometry
NFIA / Nuclear Factor 1 Antibody - Anti-NFIA antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
NFIA / Nuclear Factor 1 Antibody (aa348‑397, Biotin) LS‑C428417
Species: Human, Chimpanzee, Orangutan, Gibbon, Monkey, Mouse, Rat, Bovine, Guinea pig, Horse, Pig, Rabbit, Chicken, Xenopus
Applications: IHC, IHC - Paraffin, Western blot
Product
OPRM1 / Mu Opioid Receptor Antibody (aa161‑187, APC) LS‑C265006
Species: Human, Mouse
Applications: IHC, Western blot, Flow Cytometry, ELISA
Product
Human FASLG / Fas Ligand Protein (Recombinant DDK (Flag)) (aa103-281) - LS-G3868
Source: Human
Tag: DDK (Flag)
See all NFIA / Nuclear Factor 1 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/25/2024
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