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Anti-NFIA / Nuclear Factor 1 Antibody (aa348-397) IHC-plus™ LS-B1549

Note: This antibody replaces LS-C30109

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl - $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Most Popular NFIA / Nuclear Factor 1 Antibodies

Anti-NFIA / Nuclear Factor 1 Antibody (aa348-397) IHC-plus™ LS-B1549
Rabbit Polyclonal (IgG) to Human NFIA / Nuclear Factor 1
Human, Monkey, Mouse, Rat, Bovine, Guinea pig, Horse, Pig, Rabbit, Chicken, Xenopus
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-NFIA / Nuclear Factor 1 Antibody (aa470-498) IHC-plus™ LS-B9077
Rabbit Polyclonal to Human NFIA / Nuclear Factor 1
Human
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image
Anti-NFIA / Nuclear Factor 1 Antibody (N-Terminus) LS-C354251
Rabbit Polyclonal to Human NFIA / Nuclear Factor 1
Human, Mouse, Rat, Bovine, Pig, Chicken, Zebrafish
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human NFIA / Nuclear Factor 1
Human, Monkey, Mouse, Rat, Bovine, Guinea pig, Horse, Pig, Rabbit, Chicken, Xenopus
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human NFIA / Nuclear Factor 1
Rabbit
Human, Monkey, Mouse, Rat, Bovine, Guinea pig, Horse, Pig, Rabbit, Chicken, Xenopus (tested or 100% immunogen sequence identity)
Zebrafish (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with FITC, Biotin, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

NFIA / Nuclear Factor 1 antibody was raised against synthetic peptide located between aa348-397 of human NFIA (Q8TA97, NP_005586). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Xenopus (100%); Zebrafish (92%).
LS-E11498 - Lyophilized - 100 µg - $145.00
aa348-397
Human NFIA
Immunohistochemistry: LS-B1549 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1549 was determined to be 5 ug/ml.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl After adding water, will consist of PBS buffer with 2% sucrose
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About NFIA / Nuclear Factor 1

Q12857 NM_005595 NP_005586.1

NFIA Antibody, CTF Antibody, NF1-A Antibody, NF-I/A Antibody, NFI-L Antibody, Nuclear Factor 1 Antibody, TGGCA-binding protein Antibody, NFI-A Antibody, Nuclear factor 1 A-type Antibody, KIAA1439 Antibody, Nuclear factor 1/A Antibody, Nuclear factor I/A Antibody

Nuclear factor I (NFI) proteins constitute a family of dimeric DNA-binding proteins with similar, and possibly identical, DNA-binding specificity. They function as cellular transcription factors and as replication factors for adenovirus DNA replication. Diversity in this protein family is generated by multiple genes, differential splicing, and heterodimerization.

Immunohistochemistry

Immunohistochemistry
Anti-NFIA antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1549 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
Anti-NFIA antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1549 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
NFIA antibody LS-B1549 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
A: Marker, B: Jurkat Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/7/2016

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