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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NFI / NFIC Antibody (aa184‑233) LS‑C31022
NFIC antibody LS-C31022 is an unconjugated rabbit polyclonal antibody to NFIC (NFI) from human and monkey. Validated for WB.
Catalog
Size
Price
LS-C31022-100
100 µg (1 mg/ml)
Unavailable

Popular NFI / NFIC Products

Anti-NFIC antibody IHC of normal human adrenal (adipocytes). Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4029 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Pig
Applications: IHC, IHC - Paraffin, Western blot
Immunofluorescence of monoclonal antibody to NFIC on HeLa cell. [antibody concentration 10 ug/ml]
Species: Human
Applications: Immunofluorescence, Western blot, ELISA
Fluorescent confocal image of HeLa cell stained with NFIC Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NFIC primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NFIC immunoreactivity is localized to nucleus significantly.
Species: Human
Applications: Immunofluorescence, Western blot
Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-NFIC mouse monoclonal antibody (dilution 1:200).
Species: Human
Applications: ICC, Western blot
Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-NFIC mouse mAb (dilution 1:200).
Species: Human
Applications: ICC, Western blot

Product Description

NFIC antibody LS-C31022 is an unconjugated rabbit polyclonal antibody to NFIC (NFI) from human and monkey. Validated for WB.
About NFI / NFIC
Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication. P08651 NM_005597 NP_005588.2

NFIC Antibody, CTF Antibody, CTF5 Antibody, NF1-C Antibody, NF-I/C Antibody, NFI Antibody, NFI-C Antibody, TGGCA-binding protein Antibody, NF-I Antibody, Nuclear factor 1 C-type Antibody, Nuclear factor 1/C Antibody, Nuclear factor I/C Antibody


Specifications

Target
Human NFI / NFIC
Host
Rabbit
Reactivity
Human, Monkey (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot (2.5 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
NFI / NFIC antibody was raised against synthetic peptide located between aa184-233 of human NFIC (P08651, NP_005588). Percent identity by BLAST analysis: Human, Chimpanzee, Monkey (100%).
Epitope
aa184-233
Specificity
Human NFIC
Presentation
Lyophilized.
Reconstitution
100 µl After adding water, will consist of PBS buffer with 2% sucrose
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

A: Marker, B: HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
A: Marker, B: HepG2 Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

A: Marker, B: HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
A: Marker, B: HepG2 Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

A: Marker, B: HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
A: Marker, B: HepG2 Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

A: Marker, B: HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
A: Marker, B: HepG2 Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/18/2018

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