Products
Research Areas
COVID-19
Resources
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us

Locations


Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464


Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers



Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

Contact Us



Additional Contact Details

Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-B3051-50 50 µl (1 mg/ml) $460 
NFATC1 / NFAT2 Antibody - Anti-NFATC1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
NFATC1 / NFAT2 Antibody - IF (ICC) notes: ICC/IF analysis of NFATc1 in HaLe cells. The cell was stained with NFATc1 antibody (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
NFATC1 / NFAT2 Antibody - The cell lysates (20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NFATc1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: 293T cell lysateLane 2.: NFATc1 transfected 293T cell lysate
NFATC1 / NFAT2 Antibody - Anti-NFATC1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
NFATC1 / NFAT2 Antibody - IF (ICC) notes: ICC/IF analysis of NFATc1 in HaLe cells. The cell was stained with NFATc1 antibody (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
NFATC1 / NFAT2 Antibody - The cell lysates (20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NFATc1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: 293T cell lysateLane 2.: NFATc1 transfected 293T cell lysate
1 of 3
2 of 3
3 of 3

IHC‑plus™ Monoclonal Mouse anti‑Human NFATC1 / NFAT2 Antibody (clone AT1C3, aa428‑716, IHC, IF, WB) LS‑B3051

IHC‑plus™ Monoclonal Mouse anti‑Human NFATC1 / NFAT2 Antibody (clone AT1C3, aa428‑716, IHC, IF, WB) LS‑B3051

Note: This antibody replaces LS-C93827
Antibody:
NFATC1 / NFAT2 Mouse anti-Human Monoclonal (aa428-716) (AT1C3) Antibody
Application:
IHC, IHC-P, IF, WB, ELISA, SDS-PAGE
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B3051-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
NFATC1 / NFAT2 Mouse anti-Human Monoclonal (aa428-716) (AT1C3) Antibody
Application:
IHC, IHC-P, IF, WB, ELISA, SDS-PAGE
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
NFAT2 antibody LS-B3051 is an unconjugated mouse monoclonal antibody to human NFAT2 (NFATC1) (aa428-716). Validated for ELISA, IF, IHC, SDS-PAGE and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human NFATC1 / NFAT2
Synonyms
NFATC1 | NFATc | NFAT2 | NF-ATC | NF-ATC transcription factor | NF-ATc1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal
Clone
AT1C3
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Recombinant human NFATc1 (428-716aa) purified from E. coli. NCBI Accession No.: NP_765978.
Epitope
aa428-716
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Immunofluorescence
  • Western blot (1:1000 - 1:2000)
  • ELISA
  • Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B3051 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3051 was determined to be 10 ug/ml.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 10% Glycerol
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NFATC1 / NFAT2
O95644 NM_006162 NP_006153.2

Validation

NFATC1 / NFAT2 Antibody - Anti-NFATC1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-NFATC1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-NFATC1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

LSBio Ratings

IHC-plus™ NFATC1 / NFAT2 Antibody (aa428-716, clone AT1C3) for IHC, IF/Immunofluorescence, WB/Western, ELISA LS-B3051 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/24/2024