Merlin antibody LS-C8995 is an unconjugated rabbit polyclonal antibody to Merlin (NF2) from human and mouse. Validated for ELISA and WB.
Human NF2 / Merlin
(tested or 100% immunogen sequence identity)
- Western blot (1:1000)
- ELISA (1:1000 - 1:4000)
NF2 / Merlin antibody was raised against human Merlin (NF2) phosphorylated peptide corresponding to a region of the human protein surrounding Ser518; labeled with KLH.
Recognizes human Merlin (NF2) phosphorylated at Ser518, Mr 75kD. Specific for the phosphorylated form of human Merlin (NF2). Less than 5% cross-reactivity was detected against the non-phosphorylated form of the immunizing peptide. Species cross-reactivity: Mouse.
Suitable for use in ELISA and Western Blot. ELISA: 1:1000-1:4000. Use 0.1 ug phosphorylated peptide in a standard capture ELISA using TMB as a substrate for 30 minutes at RT. Western Blot: 1:1000. Positive control: WB: Mouse cardiac myocyte lysate.
PBS, pH 7.2, 0.01% Sodium Azide, No stabilizing proteins added
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.