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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NF2 / Merlin Antibody (phospho‑Ser518) LS‑C8995
Merlin antibody LS-C8995 is an unconjugated rabbit polyclonal antibody to Merlin (NF2) from human and mouse. Validated for ELISA and WB.
Catalog
Size
Price
LS-C8995-100
100 µg
$740
Description
Merlin antibody LS-C8995 is an unconjugated rabbit polyclonal antibody to Merlin (NF2) from human and mouse. Validated for ELISA and WB.
Target
Human NF2 / Merlin
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1:1000)
  • ELISA (1:1000 - 1:4000)
Immunogen
NF2 / Merlin antibody was raised against human Merlin (NF2) phosphorylated peptide corresponding to a region of the human protein surrounding Ser518; labeled with KLH.
Epitope
pSer518
Specificity
Recognizes human Merlin (NF2) phosphorylated at Ser518, Mr 75kD. Specific for the phosphorylated form of human Merlin (NF2). Less than 5% cross-reactivity was detected against the non-phosphorylated form of the immunizing peptide. Species cross-reactivity: Mouse.
Usage
Suitable for use in ELISA and Western Blot. ELISA: 1:1000-1:4000. Use 0.1 ug phosphorylated peptide in a standard capture ELISA using TMB as a substrate for 30 minutes at RT. Western Blot: 1:1000. Positive control: WB: Mouse cardiac myocyte lysate.
Presentation
PBS, pH 7.2, 0.01% Sodium Azide, No stabilizing proteins added
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only.
About NF2 / Merlin
P35240 NM_000268 NP_000259.1

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Requested From: United States
Date Requested: 12/15/2018
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