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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NF2 / Merlin Antibody (phospho‑Ser518) LS‑C8995
Merlin antibody LS-C8995 is an unconjugated rabbit polyclonal antibody to Merlin (NF2) from human and mouse. Validated for ELISA and WB.
Antibody
Catalog
Size
Price
LS-C8995-100
100 µg
$740

Popular NF2 / Merlin Products

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Anti-NF2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2336 concentration 5 ug/ml.
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Merlin Antibody IHC of formalin-fixed and paraffin-embedded human lung carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.
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Immunohistochemical analysis of Merlin (pS518) staining in human muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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Detection of Human NF2 by Immunohistochemistry. Sample: FFPE section of human pancreatic islet cell tumor. Antibody: Affinity purified rabbit anti- NF2 used at a dilution of 1:1000 (1 Detection: DAB.
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Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation

Product Description

Merlin antibody LS-C8995 is an unconjugated rabbit polyclonal antibody to Merlin (NF2) from human and mouse. Validated for ELISA and WB.
About NF2 / Merlin
Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. P35240 NM_000268 NP_000259.1

NF2 Antibody, ACN Antibody, BANF Antibody, Moesin-ezrin-radixin like Antibody, Neurofibromin 2 (merlin) Antibody, Neurofibromin-2 Antibody, SCH Antibody, Merlin Antibody, Schwannomerlin Antibody, Schwannomin Antibody


Specifications

Target
Human NF2 / Merlin
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1:1000)
  • ELISA (1:1000 - 1:4000)
Immunogen
NF2 / Merlin antibody was raised against human Merlin (NF2) phosphorylated peptide corresponding to a region of the human protein surrounding Ser518; labeled with KLH.
Epitope
pSer518
Specificity
Recognizes human Merlin (NF2) phosphorylated at Ser518, Mr 75kD. Specific for the phosphorylated form of human Merlin (NF2). Less than 5% cross-reactivity was detected against the non-phosphorylated form of the immunizing peptide. Species cross-reactivity: Mouse.
Usage
Suitable for use in ELISA and Western Blot. ELISA: 1:1000-1:4000. Use 0.1 ug phosphorylated peptide in a standard capture ELISA using TMB as a substrate for 30 minutes at RT. Western Blot: 1:1000. Positive control: WB: Mouse cardiac myocyte lysate.
Presentation
PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added.
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: United States
Date Requested: 7/21/2018

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