Chicken Neuron Cell Surface Antigen A2b5

Mouse

Chicken, Human (tested or 100% immunogen sequence identity)

IgM Monoclonal

Unconjugated

Purified

Unmodified

Chick retina cells.

Recognizes chicken Neuron Cell Surface Antigen A2B5. Reacts with a surface antigen on neurons in the retina, brain, spinal cord and dorsal root ganglia and with all tested human neuroblastoma cell lines. Does not seem to bind with any leukemic cell lines or with bone marrow cells from patients with leukemia, nor with cells from normal bone marrow. Cytotoxic to neurons in the presence of guinea-pig complement. Protein A binding is positive. Species cross-reactivity: human.

• IHC
• IHC - Frozen
• ICC (1:100 - 1:500)
• Immunofluorescence (1:100 - 1:500)
• Flow Cytometry

Suitable for use in Immunofluorescence, Flow Cytometry, Immunohistochemistry and Immunocytochemistry. Indirect Immunofluorescence: 1:100-1:500. For the detection of neurons in tissue culture. Using double immunofluorescence the GQ ganglioside has a similar distribution on neurons to the D2 protein, to the tetanus toxin receptor and to neurofilaments. Immunohistochemistry: Frozen, fixed tissues. Immunocytochemistry: Confocal fluorescent analysis of PC12 cells. Positive control: Type II astrocytes, human neural progenitors, PC12 cells.

PBS, pH 8.0, 0.05% sodium azide.

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.