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| Catalog Number | Size | Price |
|---|---|---|
| LS-B217-0.2 | 0.2 ml (1 mg/ml) | $460 |
IHC‑plus™ Monoclonal Mouse anti‑Human Neurofibromin / NF1 Antibody (clone McNFn27, aa27‑41, IHC) LS‑B217
IHC‑plus™ Monoclonal Mouse anti‑Human Neurofibromin / NF1 Antibody (clone McNFn27, aa27‑41, IHC) LS‑B217
Note: This antibody replaces LS-C2719
Antibody:
Neurofibromin / NF1 Mouse anti-Human Monoclonal (aa27-41) (McNFn27) Antibody
Application:
IHC, IHC-P, IP, ELISA
Reactivity:
Human, Mouse, Rat, Guinea pig
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
Neurofibromin / NF1 Mouse anti-Human Monoclonal (aa27-41) (McNFn27) Antibody
Application:
IHC, IHC-P, IP, ELISA
Reactivity:
Human, Mouse, Rat, Guinea pig
Format:
Unconjugated, Unmodified
Specifications
Description
NF1 antibody LS-B217 is an unconjugated mouse monoclonal antibody to NF1 (Neurofibromin) (aa27-41) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IHC and IP. Tested on 20 paraffin-embedded human tissues.
Target
Human Neurofibromin / NF1
Synonyms
NF1 | VRNF | Neurofibromin 1 | NFNS | WSS | Neurofibromin
Host
Mouse
Reactivity
Human, Mouse, Rat, Guinea pig
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Clone
McNFn27
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
A highly conserved N-Terminus peptide, corresponding to amino acids 27-41 of human neurofibromin
Epitope
aa27-41
Specificity
Neurofibromin (McNFn27b).
Applications
- IHC
- IHC - Paraffin (10 - 20 µg/ml)
- Immunoprecipitation
- ELISA
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B217 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B217 was determined to be 10-20 ug/ml.
Presentation
PBS, 0.05% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About Neurofibromin / NF1
Validation
Anti-NF1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-NF1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
See More About...
LSBio Ratings
IHC-plus™ Neurofibromin / NF1 Antibody (aa27-41, clone McNFn27) for IHC, IP, ELISA LS-B217 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Immunofluorescence
Species:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Pig, Chicken
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot
![Neurofibromin / NF1 Antibody - Specificity of Neurofibromin, using Neurofibromin Antibody [McNFn27a], in Schwann cells from embryonic knockout mice, adult human nerves and neonatal rat nerves. This image was taken for the unconjugated form of this product. Other forms have not been tested.](https://lsbio-7d62.kxcdn.com//image2/neurofibromin-nf1-antibody-aa27-41-clone-mcnfn27a-ls-c2721/3186_992103.jpg)
Species:
Human, Mouse, Rat, Guinea pig
Applications:
Western blot, Immunoprecipitation, ELISA
Species:
Human, Monkey, Mouse, Rat, Pig, Chicken
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Reactivity:
Mouse
Range:
0.156-10 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
Date Requested: 4/19/2024
