Mouse NeuN

Mouse

Mouse, Rat (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

A60

Biotin. Also available Unconjugated.

Protein A purified

Unmodified

Purified cell nuclei from mouse brain.

Reacts with most neuronal cell types throughout the nervous system of mice including cerebellum, cerebral cortex, hippocampus, thalamus, spinal cord and neurons in the peripheral nervous system including dorsal root ganglia, sympathetic chain ganglia and enteric ganglia. Immunohistochemical staining is primarily in the nucleus of the neurons with lighter staining in the cytoplasm. The few cell types not reactive include Purkinje, mitral and photoreceptor cells. Species cross-reactivity: Rat. It is expected that it will also react with human, ferret, chick and salamander.

• IHC
• IHC - Paraffin
• ICC (1:10 - 1:500)
• Western blot
• Applications tested for the base form of this product only

Suitable for use in Immunohistochemistry, Western Blot and Immunocytochemistry. Immunohistochemistry: 1:100-1:2000; Works best on polyester wax embedded tissue but also works on paraffin embedded tissue at a lower working dilution. Works well with formaldehyde-based fixatives. Citric acid and microwave pretreatment has been used successfully. Staining is primarily in the nucleus of the neurons with lighter staining in the cytoplasm. Developmentally, immunoreactivity is first observed shortly after neurons have become postmitotic, no staining has been observed in proliferative zones. Western Blot: Recognizes 2-3 bands in the 46-48kD range and possibly another band at ~66kD. Immunocytochemistry: 1:10-1:500 dilution with buffer without excess protein blocks or detergents. Neurons in culture should be permeabilized with 0.1% triton X-100. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

PBS, pH 7.1, 0.09% sodium azide, 15 mg/ml BSA.

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.