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Anti-NES / Nestin Antibody (Ser1409) LS-C97685


Wt. Vol. Conc. Price
- 400 µl - $305
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular NES / Nestin Antibodies

Anti-NES / Nestin Antibody (aa1484-1500) IHC-plus™ LS-B656
Rabbit Polyclonal to Human NES / Nestin
Human, Mouse
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Immunohistochemistry Image
Anti-NES / Nestin Antibody (clone 2C1.3A11) IHC-plus™ LS-B2851
Mouse Monoclonal [clone 2C1.3A11] (IgG1) to Human NES / Nestin
IHC - Paraffin, ICC, Western blot, Immunoprecipitation, Flow Cytometry
Immunohistochemistry Image
Anti-NES / Nestin Antibody IHC-plus™ LS-B5224
Chicken Polyclonal (IgY) to Human NES / Nestin
Human, Mouse
IHC - Paraffin, ICC, Western blot
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed Publications Publications (1)
Rabbit Polyclonal to Human NES / Nestin
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry


Human NES / Nestin
Human (tested or 100% immunogen sequence identity)
Immunoaffinity purified


  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:200)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)

Specificity and Use

NES / Nestin antibody was raised against kLH-conjugated synthetic peptide between amino acids 1389-1416 from human Nestin.
LS-E6812 - Lyophilized - 100 µg - $145.00


PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

Publications (1)

Identification of a novel putative pancreatic stem/progenitor cell marker DCAMKL-1 in normal mouse pancreas. May R, Sureban SM, Lightfoot SA, Hoskins AB, Brackett DJ, Postier RG, Ramanujam R, Rao CV, Wyche JH, Anant S, Houchen CW. American journal of physiology. Gastrointestinal and liver physiology. 2010 299:G303-10. [PubMed:20522640] [PMC:PMC2928534]

About NES / Nestin

P48681 X65964 CAA46780.1

NES Antibody, Nestin Antibody, Nbla00170 Antibody

Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.


Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with Nestin Antibody (S1409), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.


Fluorescent confocal image of SY5Y cells stained with Nestin (S1409) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated Nestin (S1409) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the Nestin (S1409) immunosignal to the intermediate filaments, supported by Human Protein Atlas Data (

Western blot

Western blot
Western blot of Nestin Antibody (S1409) in HepG2 cell line lysates (35 ug/lane). NES (arrow) was detected using the purified antibody.

Flow Cytometry

Flow Cytometry
Flow cytometric of HepG2 cells using Nestin Antibody (S1409)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Requested From: United States
Date Requested: 10/28/2016

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