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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NES / Nestin Antibody LS‑C124240
Antibody
Nestin antibody LS-C124240 is an unconjugated chicken polyclonal antibody to mouse Nestin (NES). Validated for ICC, IHC and WB.
Catalog
Size
Price
LS-C124240-300
300 µg
Unavailable

Popular NES / Nestin Products

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Product Description

Nestin antibody LS-C124240 is an unconjugated chicken polyclonal antibody to mouse Nestin (NES). Validated for ICC, IHC and WB.
About NES / Nestin
Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells. P48681 X65964 CAA46780.1

NES Antibody, Nestin Antibody, Nbla00170 Antibody


Specifications

Target
Mouse NES / Nestin
Host
Chicken
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgY Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC (1:10000 - 1:20000)
  • ICC (1:10000 - 1:20000)
  • Western blot (1:20000 - 1:50000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
NES / Nestin antibody was raised against three synthetic peptides corresponding to different regions of the mouse nestin (NP_057910) (KLH). Species sequence homology: rat (NM_012987, NCBI).
Specificity
Recognizes mouse Nestin. Species sequence homology: rat. (Note that the sequences of the mouse and human nestin genes are divergent, and it is unlikely that antibodies raised against the mouse sequence would recognize human nestin.).
Usage
Suitable for use in Immunohistochemistry, Immunocytochemistry and Western Blot. Immunohistochemistry: 1:10000-1:20000 using 2% paraformaldehyde tissues. Immunocytochemistry: 1:10000-1:20000 using 2% paraformaldehyde cells. Western Blot: 1:20000-1:50000.
Presentation
PBS, pH 7.2, 0.02% sodium azide
Storage
May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/22/2018

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