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Anti-NES / Nestin Antibody (clone Rat-401, 4D4) LS-C42054


Wt. Vol. Conc. Price
100 µg - 0.5 mg/ml $585
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular NES / Nestin Antibodies

Anti-NES / Nestin Antibody (aa1484-1500) IHC-plus™ LS-B656
Rabbit Polyclonal to Human NES / Nestin
Human, Mouse
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Immunohistochemistry Image
Anti-NES / Nestin Antibody (clone 2C1.3A11) IHC-plus™ LS-B2851
Mouse Monoclonal [clone 2C1.3A11] (IgG1) to Human NES / Nestin
IHC - Paraffin, ICC, Western blot, Immunoprecipitation, Flow Cytometry
Immunohistochemistry Image
Anti-NES / Nestin Antibody IHC-plus™ LS-B5224
Chicken Polyclonal (IgY) to Human NES / Nestin
Human, Mouse
IHC - Paraffin, ICC, Western blot
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone Rat-401, 4D4] (IgG1) to Rat NES / Nestin
Rat, Mouse
IHC - Frozen, Western blot


Rat NES / Nestin
Rat, Mouse (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [Rat-401, 4D4]


  • IHC - Frozen (1:20 - 1:200)
  • Western blot

  • IHC - Paraffin

Specificity and Use

NES / Nestin antibody was raised against nestin purified from embryonic rat spinal cord.
Recognizes rat nestin, a large intermediate filament protein transiently expressed in embryonic glial cells. It is predominately expressed in stem cells of the developing nervous system. Terminal differentiation is associated with a loss of nestin expression. Nestin expression has also been noted in other embryonic tissues, also in most Glioblastoma multiformes and many melanomas.
Immunohistochemistry: We recommend perfusing tissues with 4% paraformaldehyde at pH 7.4 for light microscopy or with either 4% paraformaldehyde at pH 10.0 or 4% paraformaldehyde with 0.1% glutaraldehyde at pH 7.4 for EM. For Immunocytochemistry we recommend using cells fixed in 4% paraformaldehyde buffered with 50 mM sodium borate at pH 9.5. Western Blot: LS-C42054 reacts with a band at 200-220 kD in reducing gels of newborn rat or mouse cell extracts. For the purpose of western blotting it is recommended that samples should be boiled in 4 volumes of 125 mM Tris, pH 6.8, 10% 2-mercaptoethanol, 10% glycerol and 4.6% SDS. Membranes should be blocked with milk or BSA. 5% PAGE gels are suggested.


PBS, 0.09% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
For research use only.

About NES / Nestin

P48681 X65964 CAA46780.1

NES Antibody, Nestin Antibody, Nbla00170 Antibody

Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.

Requested From: United States
Date Requested: 10/25/2016

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