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Catalog Number Size Price
LS-C204544-100 100 µl (1 mg/ml) $376 
NES / Nestin Antibody - Mixed cultures of neonatal rat neurons and glia stained with Nestin antibody (red), chicken antibody to vimentin (green) and DNA (DAPI stain, blue). Astrocytes and neuronal stem cells stain strongly and specifically in a clearly filamentous fashion with the Nestin antibody antibody. The filamentous staining pattern is as expected as both Nestin and vimentin are components of 10nm filaments. Note that some cells contain Nestin, but do not stain strongly for vimentin and so appear red. Others contain vimentin and not Nestin and so appear green- these are likely to be fibroblastic or endothelial cells. Some cells express both proteins and so appear yellowish. The presence of nestin indicates that the cells are developing astrocytes, neuroblasts or undifferentiated neural stem cells.
NES / Nestin Antibody - Western blot in of developing rat brain (P18) homogenate probe with Nestin antibody. A single strong band running at ~240kDa is seen.
NES / Nestin Antibody - Mixed cultures of neonatal rat neurons and glia stained with Nestin antibody (red), chicken antibody to vimentin (green) and DNA (DAPI stain, blue). Astrocytes and neuronal stem cells stain strongly and specifically in a clearly filamentous fashion with the Nestin antibody antibody. The filamentous staining pattern is as expected as both Nestin and vimentin are components of 10nm filaments. Note that some cells contain Nestin, but do not stain strongly for vimentin and so appear red. Others contain vimentin and not Nestin and so appear green- these are likely to be fibroblastic or endothelial cells. Some cells express both proteins and so appear yellowish. The presence of nestin indicates that the cells are developing astrocytes, neuroblasts or undifferentiated neural stem cells.
NES / Nestin Antibody - Western blot in of developing rat brain (P18) homogenate probe with Nestin antibody. A single strong band running at ~240kDa is seen.
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Monoclonal Mouse anti‑Human NES / Nestin Antibody (clone 4D11, IHC, WB) LS‑C204544

Monoclonal Mouse anti‑Human NES / Nestin Antibody (clone 4D11, IHC, WB) LS‑C204544

Note: This antibody replaces LS-C180217, LS-B9428
Antibody:
NES / Nestin Mouse anti-Human Monoclonal (4D11) Antibody
Application:
IHC, ICC, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$376
LS-C204544-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
NES / Nestin Mouse anti-Human Monoclonal (4D11) Antibody
Application:
IHC, ICC, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Nestin antibody LS-C204544 is an unconjugated mouse monoclonal antibody to human Nestin (NES). Validated for ICC, IHC and WB.
Target
Human NES / Nestin
Synonyms
NES | Nestin | Nbla00170
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
4D11
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Immunogen
Recombinant human Nestin expressed in bacteria and highly purified. The specific region was amino acids 317 to 630 of the human protein. This is a region which is 55% identical to rat Nestin and 57% identical to mouse Nestin.
Specificity
Human Nestin
Applications
  • IHC (1:500)
  • ICC (1:500)
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For immunocytochemistry on cells in tissue culture or in tissue sections, try this antibody at about between about 1:500 using fluorescent secondary antibodies. For immunoblotting 1:5000 is recommended. Expect to see a band at ~240kDa and another much weaker at ~90kDa, apparently a breakdown product of the ~240kDa band.
Presentation
Ascites fluid.
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NES / Nestin
P48681 X65964 CAA46780.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/23/2024