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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2851-0.05 | 0.05 ml (1 mg/ml) | $485 |
IHC‑plus™ Monoclonal Mouse anti‑Human NES / Nestin Antibody (clone 2C1.3A11, IHC, WB) LS‑B2851
IHC‑plus™ Monoclonal Mouse anti‑Human NES / Nestin Antibody (clone 2C1.3A11, IHC, WB) LS‑B2851
Note: This antibody replaces LS-C82299
Antibody:
NES / Nestin Mouse anti-Human Monoclonal (2C1.3A11) Antibody
Application:
IHC, IHC-P, ICC, WB, IP, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
NES / Nestin Mouse anti-Human Monoclonal (2C1.3A11) Antibody
Application:
IHC, IHC-P, ICC, WB, IP, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
Nestin antibody LS-B2851 is an unconjugated mouse monoclonal antibody to human Nestin (NES). Validated for Flow, ICC, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Clone
2C1.3A11
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
A 150 AA fragment from the cloned human Nestin conjugated to GST
Specificity
Human Nestin with no cross- reactivity to rodent
Applications
- IHC
- IHC - Paraffin (20 µg/ml)
- ICC (1:100 - 1:500)
- Western blot (1:1000 - 1:5000)
- Immunoprecipitation
- Flow Cytometry
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B2851 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2851 was determined to be 20 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About NES / Nestin
Validation
Anti-Nestin antibody IHC of human prostate, nerve. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
Anti-Nestin antibody IHC of human prostate, nerve. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ NES / Nestin Antibody (clone 2C1.3A11) for IHC, ICC, WB/Western, IP, Flow LS-B2851 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Rat
Applications:
IHC, IHC - Frozen, Immunofluorescence, Western blot
Species:
Rat, Mouse
Applications:
IHC, IHC - Frozen, ICC, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/24/2024
Date Requested: 4/24/2024
