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Anti-NES / Nestin Antibody (aa1464-1614) IHC-plus™ LS-B678

Note: This antibody replaces LS-C2578

Ordering

Wt. Vol. Conc. Price
- 100 µl 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular NES / Nestin Antibodies

Anti-NES / Nestin Antibody (aa1484-1500) IHC-plus™ LS-B656
Rabbit Polyclonal to Human NES / Nestin
Human, Mouse
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-NES / Nestin Antibody IHC-plus™ LS-B5224
Chicken Polyclonal (IgY) to Human NES / Nestin
Human, Mouse
IHC - Paraffin, ICC, Western blot
Unconjugated
Immunohistochemistry Image
Anti-NES / Nestin Antibody (clone 2C1.3A11) IHC-plus™ LS-B2851
Mouse Monoclonal [clone 2C1.3A11] (IgG1) to Human NES / Nestin
Human
IHC - Paraffin, ICC, Western blot, Immunoprecipitation, Flow Cytometry
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human NES / Nestin
Human
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human NES / Nestin
Rabbit
Human (tested or 100% immunogen sequence identity)
Mouse, Rat, Rabbit
Polyclonal
Unconjugated. Also available conjugated with Biotin, DY650, DY488, DY550.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:500 - 1:2000)
  • (applications tested for the base form of this product only)

Specificity and Use

NES / Nestin antibody was raised against recombinant human nestin protein (corresponding to residues 1464-1614)
aa1464-1614
Human nestin.
Immunohistochemistry: LS-B678 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B678 was determined to be 5 ug/ml.

Packaging

Tris-citrate/phosphate [pH 7-8], 0.1% sodium azide.
Store at 4°C. Do not freeze.
For research use only.

About NES / Nestin

P48681 X65964 CAA46780.1

NES Antibody, Nestin Antibody, Nbla00170 Antibody

Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.

Immunohistochemistry

Immunohistochemistry
Anti-Nestin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B678 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
Anti-Nestin antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B678 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/9/2016

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