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Anti-NES / Nestin Antibody (aa1464-1614, clone 10C2, DY488) LS-C142113

Catalog Size Price
LS-C142113-100 100 µl Unavailable

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134 NES / Nestin Antibodies

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Anti-NES / Nestin Antibody LS-C25743
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IHC, IHC - Frozen, ICC, Western blot
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Anti-NES / Nestin Antibody (aa1484-1500) IHC-plus™ LS-B656
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Anti-Nestin antibody IHC of mouse kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B656 concentration 5 ug/ml.
Anti-NES / Nestin Antibody (clone Rat-401, 4D4) LS-C42054
Mouse Monoclonal [clone Rat-401, 4D4] (IgG1) to Rat NES / Nestin
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Anti-NES / Nestin Antibody (clone 2C1.3A11) IHC-plus™ LS-B2851
Mouse Monoclonal [clone 2C1.3A11] (IgG1) to Human NES / Nestin
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Anti-Nestin antibody IHC of human prostate, nerve. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2851 concentration 20 ug/ml.
Anti-NES / Nestin Antibody IHC-plus™ LS-B5224
Chicken Polyclonal (IgY) to Human NES / Nestin
Human, Mouse
IHC, IHC - Paraffin, ICC, Western blot
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Anti-Nestin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5224 concentration 5 ug/ml.

100% Guaranteed
Mouse Monoclonal [clone 10C2] (IgG1,k) to Human NES / Nestin
Human
IHC, IHC - Paraffin, IHC - Frozen, ICC, Immunofluorescence, Western blot (applications tested for the base form of this product only)
DY488 Conjugated

Details

Human NES / Nestin
Mouse
Human (tested or 100% immunogen sequence identity)
Mouse, Rat, Rabbit
IgG1,k Monoclonal [10C2]
DyLight 488. Also available conjugated with Biotin, DY650, DY550.
Protein G purified
Unmodified

Applications

  • IHC
  • IHC - Paraffin
  • IHC - Frozen
  • ICC
  • Immunofluorescence (1:1000 - 1:5000)
  • Western blot (1:1000 - 1:5000)
  • (applications tested for the base form of this product only)

Specificity and Use

NES / Nestin antibody was raised against a fragment of the human nestin protein corresponding to residues 1464-1614
aa1464-1614
Human nestin.
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

50 mM sodium borate
Store at 4°C. Do not freeze.
For research use only.

About NES / Nestin

P48681 X65964 CAA46780.1

NES Antibody, Nestin Antibody, Nbla00170 Antibody

Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.

Immunohistochemistry

Anti-Nestin antibody IHC of human kidney, glomerulus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B51 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-Nestin antibody IHC of human kidney, glomerulus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B51 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunocytochemistry

Visualization of nestin in PC-3 cells. Courtesy of Dolnick Lab, Roswell Park Cancer Institute.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Visualization of nestin in PC-3 cells. Courtesy of Dolnick Lab, Roswell Park Cancer Institute. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Nestin Antibody (10C2) - Detection of human nestin.  Lane 1: 5 ug total rat brain protein, lane 2: 5 ug human CNS progenitor cell protein.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Nestin Antibody (10C2) - Detection of human nestin. Lane 1: 5 ug total rat brain protein, lane 2: 5 ug human CNS progenitor cell protein. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of human nestin. Lane 1: 5 ug total rat brain protein, lane 2: 5 ug human CNS progenitor cell protein.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of human nestin. Lane 1: 5 ug total rat brain protein, lane 2: 5 ug human CNS progenitor cell protein. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Flow Cytometry: Nestin Antibody (10C2) - FACS staining of NTERA-2 cells3 at a 1:50 dilution detected using Dylight-488 conjugated goat anti-mouse IgG secondary antibody.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Flow Cytometry: Nestin Antibody (10C2) - FACS staining of NTERA-2 cells3 at a 1:50 dilution detected using Dylight-488 conjugated goat anti-mouse IgG secondary antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 5/21/2018

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