Products
Research Areas
COVID-19
Resources
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us

Locations


Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464


Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers



Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

Contact Us



Additional Contact Details

Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order

NELFB / COBRA1

negative elongation factor complex member B

Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. May be able to induce chromatin unfolding.

Gene Name: negative elongation factor complex member B
Synonyms: NELFB, Cofactor of BRCA1, COBRA1, RP13-122B23.3, KIAA1182, Negative elongation factor B, NELF-B
Target Sequences: NM_015456 NP_056271.2 Q8WX92

☰ Filters
Antibodies (19)
Proteins (5)
Over-Expression Lysate (2)
Primary (19)
Recombinant (3)
NELFB / COBRA1 (24)
Human (19)
Mouse (4)
Rat (3)
IHC (6)
WB (18)
ChrIP (4)
ELISA (12)
IF (2)
IP (3)
Peptide-ELISA (1)
rabbit (18)
mouse (1)
IgG (17)
monoclonal mc (1)
polyclonal pc (18)
F7E4 (1)
APC Conjugated (1)
Biotin Conjugated (2)
FITC Conjugated (2)
HRP Conjugated (2)
PE Conjugated (1)
Unconjugated (11)
aa256-285 (7)
aa530-580 (2)
aa25-75 (1)
No (24)
GST (2)
Myc-DDK (Flag) (3)
Human (4)
293T Cells (1)
E. coli (1)
HEK 293 Cells (2)
Wheat Germ Extract (1)
Purified (1)
NELFB / COBRA1 Antibody - Immunohistochemistry of paraffin-embedded human colon cancer using NELFB Antibody at dilution of 1:100
Select
NELFB / COBRA1 Rabbit anti-Human Polyclonal Antibody
Human
ChrIP, ELISA, IHC, WB
Unconjugated
50 µg/$294; 100 µg/$360
Select
NELFB / COBRA1 Rabbit anti-Human Polyclonal (HRP) Antibody
Human
ChrIP, ELISA, IHC, WB
HRP Conjugated
50 µg/$294; 100 µg/$360
Select
NELFB / COBRA1 Rabbit anti-Human Polyclonal (Biotin) Antibody
Human
ChrIP, ELISA, IHC, WB
Biotin Conjugated
50 µg/$294; 100 µg/$360
Select
NELFB / COBRA1 Rabbit anti-Human Polyclonal (FITC) Antibody
Human
ChrIP, ELISA, IHC, WB
FITC Conjugated
50 µg/$294; 100 µg/$360
Select
NELFB / COBRA1 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IF, IP, WB
Unconjugated
100 µg/$331
NELFB / COBRA1 Antibody - Detection of Human COBRA1 by Immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-COBRA1 used at a dilution of 1:250. Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte Plus 555 (A120-501E) used at a dilution of 1:100.
Select
NELFB / COBRA1 Rabbit anti-Human Polyclonal (aa530-580) Antibody
Human
IF, IHC
Unconjugated
2.5 µg/$295; 100 µl/$532
NELFB / COBRA1 Antibody - Detection of Human COBRA1 by Immunohistochemistry. Sample: FFPE section of human placenta. Antibody: Affinity purified rabbit anti-COBRA1 used at a dilution of 1:200 (1 ug/ml).
Select
NELFB / COBRA1 Rabbit anti-Human Polyclonal (aa530-580) Antibody
Human
IHC, IP, WB
Unconjugated
10 µl/$295; 100 µl/$514
Select
NELFB / COBRA1 Rabbit anti-Human Polyclonal (aa256-285) Antibody
Human
ELISA, WB
Unconjugated
200 µl/$734
Select
NELFB / COBRA1 Rabbit anti-Human Polyclonal (aa256-285) (HRP) Antibody
Human
ELISA, WB
HRP Conjugated
200 µl/$952
Select
NELFB / COBRA1 Rabbit anti-Human Polyclonal (aa256-285) (Biotin) Antibody
Human
ELISA, WB
Biotin Conjugated
200 µl/$952
Select
NELFB / COBRA1 Rabbit anti-Human Polyclonal (aa256-285) (FITC) Antibody
Human
ELISA, WB
FITC Conjugated
200 µl/$952
Select
NELFB / COBRA1 Rabbit anti-Human Polyclonal (aa256-285) (APC) Antibody
Human
ELISA, WB
APC Conjugated
200 µl/$952
Select
NELFB / COBRA1 Rabbit anti-Human Polyclonal (aa256-285) (PE) Antibody
Human
ELISA, WB
PE Conjugated
200 µl/$952
NELFB / COBRA1 Antibody - Western blot analysis of COBRA1 using anti-COBRA1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: mouse kidney tissue lysates, Lane 3: human MDA-MB-231 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COBRA1 antigen affinity purified polyclonal antibody at 0.5 ?g/mL overnight at 4?C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for COBRA1 at approximately 66KD. The expected band size for COBRA1 is at 66KD.
Select
NELFB / COBRA1 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ELISA, WB
Unconjugated
10 µg/$318; 100 µg/$470
Select
NELFB / COBRA1 Rabbit anti-Human Polyclonal (aa25-75) Antibody
Human
IP, WB
Unconjugated
100 µl/$467
NELFB / COBRA1 Antibody - Western blot analysis of extracts of HepG2 cells using NELFB antibody. The lane on the left was treated with blocking peptide.
Select
NELFB / COBRA1 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
NELFB / COBRA1 Antibody
Select
NELFB / COBRA1 Mouse anti-Human Monoclonal (F7E4) Antibody
Mouse, Rat, Human
WB
Unconjugated
100 µl/$469
NELFB / COBRA1 Antibody - COBRA1 Antibody western blot of 293 cell line lysates (35 ug/lane). The COBRA1 antibody detected the COBRA1 protein (arrow).
Select
NELFB / COBRA1 Rabbit anti-Human Polyclonal (aa256-285) Antibody
Human
WB
Unconjugated
400 µl/$393
NELFB / COBRA1 Antibody - Western blot analysis of extracts of various cell lines, using NELFB antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 5s.
Select
NELFB / COBRA1 Rabbit anti-Human Polyclonal Antibody
Mouse, Human
WB
Unconjugated
20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503
NELFB / COBRA1 Protein - Purified recombinant protein NELFB was analyzed by SDS-PAGE gel and Coomassie Blue Staining
Select
HEK 293 Cells
Myc-DDK (Flag)
65.5 kDa
20 µg/$1,107
NELFB / COBRA1 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
293T Cells
Myc-DDK (Flag)
65.5 kDa
20 µg/$215
Select
E. coli
GST
49.2 kD
1 mg/$1,355; 100 µg/$420; 20 µg/$323
NELFB / COBRA1 Protein - 12.5% SDS-PAGE of human COBRA1 stained with Coomassie Blue
Select
Wheat Germ Extract
GST
77.99 kDa
10 µg/$479; 25 µg/$670
NELFB / COBRA1 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
65.5 kDa
100 µg/$710
Viewing 1-24 of 24 product results



If you do not find the reagent or information you require, please contact Customer.Support@LSBio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).