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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NEK7 Antibody (N‑Terminus) IHC‑plus™ LS‑A7163 Best Seller
NEK7 antibody LS-A7163 is an unconjugated rabbit polyclonal antibody to NEK7 from human, mouse, rat and other species. Validated for IHC. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-A7163-50
50 µg (1 mg/ml)
$425
In Stock for Immediate Shipment

Popular NEK7 Products

Anti-NEK7 antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8348 dilution 3.75 ug/ml.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Chicken
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
IHC of paraffin-embedded Tw2.6 xenograft using NEK7 antibody at 1:100 dilution.
Species: Human, Rat
Applications: IHC, IHC - Paraffin, ICC, Western blot
Anti-NEK7 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10926 dilution 1:50.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Detection of Human NEK7 by Immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-NEK7 used at a dilution of 1:1000 (1 Detection: DAB.
Species: Human, Bovine, Dog, Horse, Pig, Rabbit
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation

Product Description

NEK7 antibody LS-A7163 is an unconjugated rabbit polyclonal antibody to NEK7 from human, mouse, rat and other species. Validated for IHC. Tested on 20 paraffin-embedded human tissues.

Specifications

Target
Human NEK7
Synonyms
NEK7, NimA-related protein kinase 7, NIMA-related kinase 7
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Chicken (tested or 100% immunogen sequence identity)
Predicted
Xenopus (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
NEK7 antibody was raised against synthetic 16 amino acid peptide from N-Terminus of human NEK7. Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Bat, Hamster, Elephant, Panda, Horse, Rabbit, Pig, Opossum, Turkey, Chicken (100%); Xenopus (94%).
Blocking Peptide
LS-E29233 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-A7163. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
N-Terminus
Specificity
Human NEK7. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Usage
Immunohistochemistry with formalin-fixed paraffin-embedded tissues requires pretreatment with Proteinase K.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About NEK7
Protein kinase which plays an important role in mitotic cell cycle progression. Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis. Phosphorylates RPS6KB1. Q8TDX7 NM_133494 NP_598001.1


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Images

Immunohistochemistry

Anti-NEK7 antibody LS-A7163 IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NEK7 antibody LS-A7163 IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-NEK7 antibody LS-A7163 IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NEK7 antibody LS-A7163 IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-NEK7 antibody LS-A7163 IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NEK7 antibody LS-A7163 IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-NEK7 antibody LS-A7163 IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NEK7 antibody LS-A7163 IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-NEK7 antibody LS-A7163 IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NEK7 antibody LS-A7163 IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-NEK7 antibody LS-A7163 IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NEK7 antibody LS-A7163 IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-NEK7 antibody LS-A7163 IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NEK7 antibody LS-A7163 IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-NEK7 antibody LS-A7163 IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-NEK7 antibody LS-A7163 IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Requested From: United States
Date Requested: 10/20/2018

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