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Anti-NEK3 Antibody (clone 2F8) LS-C197766

Ordering

Wt. Vol. Conc. Price
100 µg - - $355
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular NEK3 Antibodies

Anti-NEK3 Antibody (aa446-474, APC) LS-C264185
Rabbit Polyclonal (IgG) to Mouse NEK3
Mouse
IHC, Western blot, ELISA
APC Conjugated
Anti-NEK3 Antibody (aa339-368, FITC) LS-C264193
Rabbit Polyclonal (IgG) to Human NEK3
Human
Western blot, ELISA
FITC Conjugated
Anti-NEK3 Antibody (aa339-368, Azide-free, HRP) LS-C264197
Rabbit Polyclonal (IgG) to Human NEK3
Human
Western blot, ELISA
HRP Conjugated, Azide-free

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 2F8] (IgG1,k) to Human NEK3
Human
Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human NEK3
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [2F8]
Unconjugated
Protein A purified
Unmodified

Applications

  • Immunofluorescence (10 µg/ml)
  • Western blot
  • ELISA

Specificity and Use

NEK3 antibody was raised against nEK3 (AAH19916, aa406-506 partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human NEK3
Sandwich ELISA: Recombinant protein. Western Blot: Transfected lysate, Recombinant protein.

Packaging

PBS, pH 7.2
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
For research use only.

About NEK3

P51956 AB072828 CAD34740.1

NEK3 Antibody, HSPK 36 Antibody, HSPK36 Antibody, NIMA-related kinase 3 Antibody, NimA-related protein kinase 3 Antibody, Hydroxyalkyl-protein kinase Antibody, Phosphorylase B kinase kinase Antibody, Glycogen synthase A kinase Antibody

Protein kinase which influences neuronal morphogenesis and polarity through effects on microtubules. Regulates microtubule acetylation in neurons. Contributes to prolactin-mediated phosphorylation of PXN and VAV2. Implicated in prolactin-mediated cytoskeletal reorganization and motility of breast cancer cells through mechanisms involving RAC1 activation and phosphorylation of PXN and VAV2.

Immunofluorescence

Immunofluorescence
Immunofluorescence of monoclonal antibody to NEK3 on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

Western blot
Western Blot analysis of NEK3 expression in transfected 293T cell line by NEK3 monoclonal antibody (M01), clone 2F8.Lane 1: NEK3 transfected lysate(57.7 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged NEK3 is approximately 0.03 ng/ml as a capture antibody.
 

Requested From: United States
Date Requested: 2/22/2017

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