Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
Monkey (at least 90% immunogen sequence identity)
ELISA (1:2000 - 1:8000)
Specificity and Use
NEK2 antibody was raised against synthetic peptide corresponding to aa 287-299 of Human NEK2 protein. Peptide Sequence: GRQLGEPEKSQDS. Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%); Monkey, Marmoset (92%).
Recognizes human NEK2.
Suitable for use in ELISA. ELISA: 1:2000-1:8000; against 0.1 ug of the immunizing peptide.
PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added.
Short term: +4°C; Long term: Add glycerol (40-50%) -20°C.
Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells. Regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, CEP250 and NINL, resulting in their displacement from the centrosomes.