NEIL3 antibody LS-B259 is an unconjugated rabbit polyclonal antibody to human NEIL3 (NEI3). Validated for IHC. Tested on 20 paraffin-embedded human tissues.
(tested or 100% immunogen sequence identity)
NEI3 / NEIL3 antibody was raised against residues 164-177 (LRAESEVKKQKGRMLG) of the human NEI3 protein. 85% sequence identity to mouse and rat. Percent identity by BLAST analysis: Human, Gorilla, Marmoset (100%); Gibbon, Monkey, Elephant (94%); Panda, Dog, Bovine, Horse (88%); Chicken (81%).
Immunohistochemistry: LS-B259 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B259 was determined to be 2.5 ug/ml.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.