Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rat, Human, Mouse, Cat, Chicken, Fish (tested or 100% immunogen sequence identity)
Ammonium sulfate precipitation
Immunofluorescence (1:2000), Western blot (1:10000)
Specificity and Use
NEFM / NF-M antibody was raised against the immunogen used to raise this particular antibody was to the C-terminal extension of rat NF-M, the so-called KE segment, was expressed in bacteria and purified from inclusion bodies by dissolving in 6 M urea followed by preparative gel electrophoresis
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
View of mixed neuron/glial cultures stained with NEFM / NF-M Antibody LS-C2722 (red). The NF-M protein is assembled into neurofilaments which are found throughout the axons, dendrites and perikarya of these cells.
160kDa Neurofilament Medium Antibody - Western blots of homogenates of SH-SY5Y cells, a human neuroblastoma cell line. Lane A shows blotting with NEFM / NF-M Antibody LS-C2722, which reveals a strong NF-M band at ~150kDa. Lanes B and C are homogenates of SH-SY5Y cells which were treated with maitotoxin to activate caspase family enzymes (ref. 2). Now a ~100kDa band is seen in addition o the major NF-M band. This corresponds to the C-terminal segment of NF-M which is an in vivo calpain degradation product of human NF-M.
Requested From: United States
Date Requested: 10/1/2016