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Anti-NEFM / NF-M Antibody (C-Terminus, clone 3H11) LS-C2728

Ordering

Wt. Vol. Conc. Price
- 50 µl - $335
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
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Mouse Monoclonal [clone 3H11] (IgG1,k) to Rat NEFM / NF-M
Rat, Human, Mouse, Avian, Mammal
IHC - Paraffin, IHC - Fixed, Western blot
Unconjugated

Details

Rat NEFM / NF-M
Mouse
Rat, Human, Mouse, Avian, Mammal (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [3H11]
Ascites

Applications

IHC - Paraffin, IHC - Fixed, Western blot (1:2500)

Specificity and Use

NEFM / NF-M antibody was raised against recombinant C-terminus of rat NF-M
C-Terminus
Specifically recognizes the medium neurofilament subunit (~145-170 kD).

Packaging

Ascites.
Long term: -70°C; Short term: +4°C
For research use only.

About NEFM / NF-M

P07197 NM_005382 NP_005373.2

NEFM Antibody, 160 kDa neurofilament protein Antibody, NEF3 Antibody, NF-M Antibody, NFM Antibody, Neurofilament 3 Antibody

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Immunofluorescence

Immunofluorescence
Culture of adult neural cells grown as described (2). Mature neurons can be identified by their morphology and because they stain strongly with antibodies to NF-L, NF-M and NF-H. The surrounding stellate red cells are stained with rabbit polyclonal Rat facial nucleus before RPCA-a-Int. These are apparently mitotic neuronal progenitor cells and express many other neuronal markers.

Western blot

Western blot
Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stained with various antibodies including NEFM / NF-M Antibody LS-C2728 (lane 3).

Requested From: United States
Date Requested: 9/26/2016

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