Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene.
Shows a section of rat spinal cord stained with NEFH / NF-H antibody (green) and monoclonal antibody to ubiquitin C-terminal hydrolase 1 (UCHL1) clone MCA-BH7 (red). The neurofilament NF-H antibody binds primarily to phosphorylated axonal forms of NF-H, and so stains axons coursing between the large UCHL1 positive neurons. These large cells are motorneurons and UCHL1 protein is a major component of the perikarya and dendrites of these cells.
Lane labeled CBB shows Coomassie Brilliant Blue stained crude extract of rat spinal cord, with the prominent major neurofilament subunit indicated. In rodents, NF-H runs at 200kDa, NF-M at 145kDa and NF-L at 68kDa. The Rab lane shows a blot probed with rabbit anti NF-H antibody NEFH / NF-H antibody. A prominent band at an apparent SDS-PAGE molecular weight of 200kDa corresponds to phosphorylated form of NF-H. The lane marked Rab shows a similar blot probed with our rabbit antibody to NF-H, CPCA-NF-H.