Human NEFH / NF-H

Chicken

Human (tested or 100% immunogen sequence identity)

IgY Polyclonal

Unconjugated

Purified

Unmodified

To raise this antibody bovine intermediate filaments were prepared from spinal cords by the glycerol polymerization method of Delacourte et al., and the cytoskeletal material was dissolved in 6M urea. Individual neurofilament subunits were purified by ion exchange chromatography on DEAE-cellulose and the NF-H containing fractions were concentrated and further purified by preparative gel electrophoresis on a Biorad Prepcell.

Human NEFH / NF-H

• ICC (1:100000)
• Immunofluorescence (1:100000)
• Western blot (1:1000000)

The IgY solution has an extremely high titre and can be used at dilutions of at least 1:100000 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:1000000 or lower. The antibody has also been used successfully in the capture role in ELISA.

Liquid

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P12036 NCBI: NM_021076 NP_066554.2