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Anti-NEFH / NF-H Antibody LS-C204556

Ordering

Wt. Vol. Conc. Price
- 50 µl - $315
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular NEFH / NF-H Antibodies

Anti-NEFH / NF-H Antibody (C-Terminus) IHC-plus™ LS-B256
Rabbit Polyclonal to Rat NEFH / NF-H
Rat, Human, Mouse, Bovine, Cat, Pig
IHC - Paraffin, IHC - Frozen, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image
Anti-NEFH / NF-H Antibody (clone NF-05) IHC-plus™ LS-B4502
Mouse Monoclonal [clone NF-05] (IgG1) to Human NEFH / NF-H
Human, Rat, Pig
IHC - Paraffin, IHC - Frozen, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-NEFH / NF-H Antibody LS-C414328
Rabbit Polyclonal to Human NEFH / NF-H
Human, Mouse, Rat
IHC, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Chicken Polyclonal (IgY) to Human NEFH / NF-H
Human
ICC, Immunofluorescence, Western blot
Unconjugated

Details

Human NEFH / NF-H
Chicken
Human (tested or 100% immunogen sequence identity)
IgY Polyclonal
Unconjugated
Purified
Unmodified

Applications

  • ICC (1:100000)
  • Immunofluorescence (1:100000)
  • Western blot (1:1000000)

Specificity and Use

NEFH / NF-H antibody was raised against to raise this antibody bovine intermediate filaments were prepared from spinal cords by the glycerol polymerization method of Delacourte et al., and the cytoskeletal material was dissolved in 6M urea. Individual neurofilament subunits were purified by ion exchange chromatography on DEAE-cellulose and the NF-H containing fractions were concentrated and further purified by preparative gel electrophoresis on a Biorad Prepcell.
Human NEFH / NF-H
The IgY solution has an extremely high titre and can be used at dilutions of at least 1:100000 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:1000000 or lower. The antibody has also been used successfully in the capture role in ELISA.

Packaging

Liquid
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About NEFH / NF-H

P12036 NM_021076 NP_066554.2

NEFH Antibody, 200 kDa neurofilament protein Antibody, KIAA0845 Antibody, NFH Antibody, NF-H Antibody

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene.

Immunocytochemistry

Immunocytochemistry
Rat mixed neuron/glial cultures stained with mouse monoclonal antibody to neurofilament subunit NF-L MCA-7D1 (green) and NEFH / NF-H antibody, rabbit antibody to neurofilament NF-H. This antibody binds primarily to the phosphorylated axonal forms of NF-H, in contrast to the NF-L antibody which stains both axonal and dendritic/perikaryal neurofilaments. The NF-L antibody therefore reveals a prominent cell body in green, while the surrounding axonal profiles are orange, since the are bound by both NF-L and the chicken NF-H antibody. Blue is a DNA stain.

Western blot

Western blot
CBB shows lane of Coomassie Brilliant Blue stained crude extract of rat spinal cord, with the prominent major neurofilament subunit indicated; In rodents, NF-H runs at ~200kDa, NF-M at ~145kDa and NF-L at ~68kDa; in larger species such as pig or human, NF-H runs somewhat slower at ~210kDa or 220kDa. The Chk lane shows a blot probed with chicken anti NF-H antibody NEFH / NF-H antibody. A prominent band at an apparent SDS-PAGE molecular weight of 200kDa corresponds to phosphorylated form of NF-H. The lane marked Rab shows a similar blot probed with our rabbit antibody to NF-H, RPCA-NF-H.

Requested From: United States
Date Requested: 12/6/2016

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