Rat NEFH / NF-H

Mouse

Rat, Human, Mouse, Pig, Chicken (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

SPM203

Unconjugated

Protein G purified

Unmodified. Also available Azide-free, BSA-free.

The Triton-X 100 insoluble protein fraction of rat brain.

Rat NEFH / NF-H

• IHC
• IHC - Paraffin (0.25 - 0.5 µg/ml)
• Flow Cytometry (0.5 - 1 µg/10E6 cells)

This mAb reacts with a 200kDa protein, identified as heavy subunit of neurofilaments. Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and cell carcinomas of the lung also express neurofilament. The optimal dilution of the anti-NF-H antibody for each application should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P12036 NCBI: NM_021076 NP_066554.2