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Catalog Number Size Price
LS-C204551-100 100 µl $376 
NEFH / NF-H Antibody - Human cerebellar cortex fixed in formalin, embedded in paraffin and stained with NEFH / NF-H antibody using the ABC (avidin biotin conjugate) method. The section was counterstained with hematoxylin-Eosin (blue). NEFH / NF-H antibody stains prominent basket cell axons surrounding the large Purkinje neurons. Granule cell layer at bottom of image, molecular layer at top.
NEFH / NF-H Antibody - Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stained with various antibodies including NEFH / NF-H antibody (lane 2).
NEFH / NF-H Antibody - Human cerebellar cortex fixed in formalin, embedded in paraffin and stained with NEFH / NF-H antibody using the ABC (avidin biotin conjugate) method. The section was counterstained with hematoxylin-Eosin (blue). NEFH / NF-H antibody stains prominent basket cell axons surrounding the large Purkinje neurons. Granule cell layer at bottom of image, molecular layer at top.
NEFH / NF-H Antibody - Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stained with various antibodies including NEFH / NF-H antibody (lane 2).
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Monoclonal Mouse anti‑Pig NEFH / NF‑H Antibody (clone NAP4, IHC, IF, WB) LS‑C204551

Monoclonal Mouse anti‑Pig NEFH / NF‑H Antibody (clone NAP4, IHC, IF, WB) LS‑C204551

Antibody:
NEFH / NF-H Mouse anti-Pig Monoclonal (NAP4) Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Pig, Chicken, Mammal
Format:
Unconjugated, Unmodified
Price
Catalog Number
$376
LS-C204551-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
NEFH / NF-H Mouse anti-Pig Monoclonal (NAP4) Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Pig, Chicken, Mammal
Format:
Unconjugated, Unmodified

Specifications

Description
NF-H antibody LS-C204551 is an unconjugated mouse monoclonal antibody to NF-H (NEFH) from pig. It is reactive with chicken and mammal. Validated for IF, IHC and WB.
Target
Pig NEFH / NF-H
Synonyms
NEFH | 200 kDa neurofilament protein | KIAA0845 | NFH | NF-H
Host
Mouse
Reactivity
Pig, Chicken, Mammal (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
NAP4
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Immunogen
Native NF-H purified from pig spinal cord.
Specificity
Clone NAP4 irecognizes phosphorylated NF-H KSP (lysine-serine-proline) type sequences. In some species there is some cross-reactivity with the related phosphorylated KSP sequences found in the related neurofilament subunit NF-M. The antibody recognizes NF-H strongly in all mammals tested to date and also in chicken. It recognizes neurofilaments in frozen sections in tissue culture and in formalin fixed sections.
Applications
  • IHC
  • IHC - Paraffin (1:1000)
  • Immunofluorescence (1:1000)
  • Western blot (1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The ascites solution has a high titre and can be used at dilutions of at least 1:1000 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:10000 or lower.
Presentation
Ascites fluid.
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NEFH / NF-H
P12036 NM_021076 NP_066554.2

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Requested From: United States
Date Requested: 4/25/2024